Human kallikrein 10 expression in surgically removed human pituitary corticotroph adenomas: an immunohistochemical study / Luis Vicente Syro Moreno  

Público ISBD Título : Human kallikrein 10 expression in surgically removed human pituitary corticotroph adenomas: an immunohistochemical study Tipo de documento : documento electrónico Autores : Luis Vicente Syro Moreno, Fecha de publicación : 2015 Títulos uniformes : Applied Immunohistochemistry and Molecular Morphology Idioma : Inglés (eng) Palabras clave : Human kallkrein 10  immunohistochemistry  neoplasms  pituitary adenoma  prognostic marker Resumen : Human kallikrein 10 (hk10), a secreted serine protease, was reported to function as a tumor suppressor. hK10 immunoexpression has been demonstrated in lactrotrophs and corticotrophs of the nontumorous human adenohypophysis. In the present study, for the first time we report hK10 immunoexpression in various surgically removed corticotroph adenoma subtypes. Specimens were fixed in formalin and embedded in paraffin. Immunostaining was performed using the streptavidin-biotin peroxidase complex method with an hK10-specific rabbit polyclonal antibody. Results showed that the endocrinologically active adrenocorticotropic hormone (ACTH)-producing pituitary tumors and the silent subtypes were immunopositve for hK10. Intensity of staining varied between the different subtypes. Intensity was lowest in the silent subtypes (silent corticotroph subtypes 1 and 2) compared with nontumorous human adenohypophysial corticotrophs, whereas the endocrinologically active subtypes (ACTH-secreting adenomas, corticotroph carcinomas, Crooke cell adenomas, Crooke cell carcinomas), showed the highest hK10 immunoexpression. Immunopositivity in the nuclei of the ACTH-secreting adenomas and carcinomas, as well as dual cytoplasmic and nuclear localization of hK10 in some of the secreting tumor types was an intriguing finding. Immunoexpression of hK10 in the ACTH-secreting tumors as well as in the Crooke cell tumors was significantly increased when compared with the nonfunctioning tumors and in the corticotrophs of nontumorous pituitaries. Mención de responsabilidad : Ashley Di Meo, Fabio Rotondo, Kalman Kovacs, Michael D Cusimano, Luis V Syro, Antonio Di Ieva, Eleftheros P Diamandis, George M Yousef Referencia : Appl Immunohistochem Mol Morphol. 2015 Jul;23(6):433-7. DOI (Digital Object Identifier) : 10.1097/PAI.0000000000000108 PMID : 25517869 En línea : https://journals.lww.com/appliedimmunohist/Abstract/2015/07000/Human_Kallikrein_ [...] Enlace permanente : https://hospitalpablotobon.cloudbiteca.com/pmb/opac_css/index.php?lvl=notice_dis Human kallikrein 10 expression in surgically removed human pituitary corticotroph adenomas: an immunohistochemical study [documento electrónico] / Luis Vicente Syro Moreno,  . - 2015. Obra : Applied Immunohistochemistry and Molecular Morphology Idioma : Inglés (eng) Palabras clave : Human kallkrein 10  immunohistochemistry  neoplasms  pituitary adenoma  prognostic marker Resumen : Human kallikrein 10 (hk10), a secreted serine protease, was reported to function as a tumor suppressor. hK10 immunoexpression has been demonstrated in lactrotrophs and corticotrophs of the nontumorous human adenohypophysis. In the present study, for the first time we report hK10 immunoexpression in various surgically removed corticotroph adenoma subtypes. Specimens were fixed in formalin and embedded in paraffin. Immunostaining was performed using the streptavidin-biotin peroxidase complex method with an hK10-specific rabbit polyclonal antibody. Results showed that the endocrinologically active adrenocorticotropic hormone (ACTH)-producing pituitary tumors and the silent subtypes were immunopositve for hK10. Intensity of staining varied between the different subtypes. Intensity was lowest in the silent subtypes (silent corticotroph subtypes 1 and 2) compared with nontumorous human adenohypophysial corticotrophs, whereas the endocrinologically active subtypes (ACTH-secreting adenomas, corticotroph carcinomas, Crooke cell adenomas, Crooke cell carcinomas), showed the highest hK10 immunoexpression. Immunopositivity in the nuclei of the ACTH-secreting adenomas and carcinomas, as well as dual cytoplasmic and nuclear localization of hK10 in some of the secreting tumor types was an intriguing finding. Immunoexpression of hK10 in the ACTH-secreting tumors as well as in the Crooke cell tumors was significantly increased when compared with the nonfunctioning tumors and in the corticotrophs of nontumorous pituitaries. Mención de responsabilidad : Ashley Di Meo, Fabio Rotondo, Kalman Kovacs, Michael D Cusimano, Luis V Syro, Antonio Di Ieva, Eleftheros P Diamandis, George M Yousef Referencia : Appl Immunohistochem Mol Morphol. 2015 Jul;23(6):433-7. DOI (Digital Object Identifier) : 10.1097/PAI.0000000000000108 PMID : 25517869 En línea : https://journals.lww.com/appliedimmunohist/Abstract/2015/07000/Human_Kallikrein_ [...] Enlace permanente : https://hospitalpablotobon.cloudbiteca.com/pmb/opac_css/index.php?lvl=notice_dis

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Ghrelin immunoexpression in the human hypophysis / Luis Vicente Syro Moreno  

Público ISBD Título : Ghrelin immunoexpression in the human hypophysis Tipo de documento : documento electrónico Autores : Luis Vicente Syro Moreno, Fecha de publicación : 2012 Títulos uniformes : Applied Immunohistochemistry and Molecular Morphology Idioma : Inglés (eng) Palabras clave : ghrelin  hormone  hypophysis  immunohistochemistry  morphology Resumen : The aim of this study was to immunohistochemically localize ghrelin in autopsy-obtained, nontumoral human pituitaries. Double immunostaining was also undertaken to determine the pituitary cell type expressing both adenohypophysial hormones and ghrelin. Results showed that ghrelin is present in the adenohypophysis, its immunoexpression being cytoplasmic, weak-to-moderate, and localized to a subset of cells. Double immunostaining showed that ghrelin is present in 51% to 90% of growth hormone-producing, luteinizing-producing, and α-subunit-producing cells. Ghrelin immunoexpression was less frequently observed in other adenohypophysial cell types, being seen in 30% of adrenocorticotropin and follicle-stimulating hormones, 15% of thyrotropin, and 10% of prolactin-immunoreactive cells. Ghrelin immunopositivity was also seen in nerve fibers and Herring bodies of the neurohypophysis and pituitary stalk. More work is needed to elucidate the role of ghrelin in adenohypophysial and neurohypophysial endocrine activity. It may well be that ghrelin exerts an autocrine/paracrine effect and can modulate hormone synthesis and release. Mención de responsabilidad : Fabio Rotondo, Angelo Rotondo, Bernd W Scheithauer, Michael Cusimano, Eleanor Latta, Luis V Syro, Kalman Kovacs Referencia : Appl Immunohistochem Mol Morphol. 2012 Jan;20(1):77-81. DOI (Digital Object Identifier) : 10.1097/PAI.0b013e3182247506 PMID : 22157058 En línea : https://journals.lww.com/appliedimmunohist/Abstract/2012/01000/Ghrelin_Immunoexp [...] Enlace permanente : https://hospitalpablotobon.cloudbiteca.com/pmb/opac_css/index.php?lvl=notice_dis Ghrelin immunoexpression in the human hypophysis [documento electrónico] / Luis Vicente Syro Moreno,  . - 2012. Obra : Applied Immunohistochemistry and Molecular Morphology Idioma : Inglés (eng) Palabras clave : ghrelin  hormone  hypophysis  immunohistochemistry  morphology Resumen : The aim of this study was to immunohistochemically localize ghrelin in autopsy-obtained, nontumoral human pituitaries. Double immunostaining was also undertaken to determine the pituitary cell type expressing both adenohypophysial hormones and ghrelin. Results showed that ghrelin is present in the adenohypophysis, its immunoexpression being cytoplasmic, weak-to-moderate, and localized to a subset of cells. Double immunostaining showed that ghrelin is present in 51% to 90% of growth hormone-producing, luteinizing-producing, and α-subunit-producing cells. Ghrelin immunoexpression was less frequently observed in other adenohypophysial cell types, being seen in 30% of adrenocorticotropin and follicle-stimulating hormones, 15% of thyrotropin, and 10% of prolactin-immunoreactive cells. Ghrelin immunopositivity was also seen in nerve fibers and Herring bodies of the neurohypophysis and pituitary stalk. More work is needed to elucidate the role of ghrelin in adenohypophysial and neurohypophysial endocrine activity. It may well be that ghrelin exerts an autocrine/paracrine effect and can modulate hormone synthesis and release. Mención de responsabilidad : Fabio Rotondo, Angelo Rotondo, Bernd W Scheithauer, Michael Cusimano, Eleanor Latta, Luis V Syro, Kalman Kovacs Referencia : Appl Immunohistochem Mol Morphol. 2012 Jan;20(1):77-81. DOI (Digital Object Identifier) : 10.1097/PAI.0b013e3182247506 PMID : 22157058 En línea : https://journals.lww.com/appliedimmunohist/Abstract/2012/01000/Ghrelin_Immunoexp [...] Enlace permanente : https://hospitalpablotobon.cloudbiteca.com/pmb/opac_css/index.php?lvl=notice_dis

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