| Título : |
Submicroscopic infection of placenta by Plasmodium produces Th1/Th2 cytokine imbalance, inflammation and hypoxia in women from north-west Colombia |
| Tipo de documento : |
documento electrónico |
| Autores : |
Beatriz Helena Aristizábal Bernal, |
| Fecha de publicación : |
2014 |
| Títulos uniformes : |
Malaria Journal
|
| Idioma : |
Inglés (eng) |
| Palabras clave : |
Submicroscopic Placental malaria Plasmodium Cytokine Inflammation Hypoxia |
| Resumen : |
Background: A large-scale study was set up in order to study the epidemiology, clinical aspects, and immunopathology of gestational and placental malaria in north-west Colombia. In this region, recent reports using a qPCR technique, confirmed frequencies of infection, by Plasmodium falciparum or Plasmodium vivax, up to 45%. Given the high rates of infection observed both in mother and placenta, a first exploratory study was proposed in order to characterize the effect on the inflammation status, tissue damage and hypoxia in Plasmodium spp. infected placentas.Methods: A descriptive, prospective, cross-sectional design was applied to pregnant women with (PM+) and without (PM-) placental malaria. Messenger RNA expression of Fas, FasL; COX-1, COX-2, HIF, VEGF, and the cytokines IL-2, IL-4, IL-10, IFN-γ and TNF, were measured in peripheral and placental blood using a quantitative PCR. The percentage of apoptotic cells was determined with a TUNEL assay. Results: In total 50 placentas were studied: 25 were positive for submicroscopic infection and 25 were negative for Plasmodium infection. Expression of IL-4 and IL-10 was observed high in placental tissue of PM+, while IL-2 was high in peripheral blood of the same group. Expression of TNF and IFNγ in peripheral blood of the PM + group was high. Similarly, the apoptotic index and Fas expression were significantly high in PM+. However, FasL expression was observed low in PM + compared to PM-. Inflammation markers (HIF, VEGF) and hypoxia markers (COX-1, COX-2) were high in the PM + group. Conclusion: During placental malaria expression of some pro-inflammatory cytokines is up-regulated and markers of hypoxia and tissue damage are increased in cases of submicroscopic infection. |
| Mención de responsabilidad : |
Olga M Agudelo, Beatriz H Aristizabal, Stephanie K Yanow, Eliana Arango, Jaime Carmona-Fonseca, Amanda Maestre |
| Referencia : |
Malar J. 2014 Mar 27;13:122. |
| DOI (Digital Object Identifier) : |
10.1186/1475-2875-13-122 |
| PMID : |
24673747 |
| Derechos de uso : |
CC BY |
| En línea : |
https://malariajournal.biomedcentral.com/articles/10.1186/1475-2875-13-122 |
| Enlace permanente : |
https://hospitalpablotobon.cloudbiteca.com/pmb/opac_css/index.php?lvl=notice_dis |
Submicroscopic infection of placenta by Plasmodium produces Th1/Th2 cytokine imbalance, inflammation and hypoxia in women from north-west Colombia [documento electrónico] / Beatriz Helena Aristizábal Bernal, . - 2014. Obra : Malaria JournalIdioma : Inglés ( eng)
| Palabras clave : |
Submicroscopic Placental malaria Plasmodium Cytokine Inflammation Hypoxia |
| Resumen : |
Background: A large-scale study was set up in order to study the epidemiology, clinical aspects, and immunopathology of gestational and placental malaria in north-west Colombia. In this region, recent reports using a qPCR technique, confirmed frequencies of infection, by Plasmodium falciparum or Plasmodium vivax, up to 45%. Given the high rates of infection observed both in mother and placenta, a first exploratory study was proposed in order to characterize the effect on the inflammation status, tissue damage and hypoxia in Plasmodium spp. infected placentas.Methods: A descriptive, prospective, cross-sectional design was applied to pregnant women with (PM+) and without (PM-) placental malaria. Messenger RNA expression of Fas, FasL; COX-1, COX-2, HIF, VEGF, and the cytokines IL-2, IL-4, IL-10, IFN-γ and TNF, were measured in peripheral and placental blood using a quantitative PCR. The percentage of apoptotic cells was determined with a TUNEL assay. Results: In total 50 placentas were studied: 25 were positive for submicroscopic infection and 25 were negative for Plasmodium infection. Expression of IL-4 and IL-10 was observed high in placental tissue of PM+, while IL-2 was high in peripheral blood of the same group. Expression of TNF and IFNγ in peripheral blood of the PM + group was high. Similarly, the apoptotic index and Fas expression were significantly high in PM+. However, FasL expression was observed low in PM + compared to PM-. Inflammation markers (HIF, VEGF) and hypoxia markers (COX-1, COX-2) were high in the PM + group. Conclusion: During placental malaria expression of some pro-inflammatory cytokines is up-regulated and markers of hypoxia and tissue damage are increased in cases of submicroscopic infection. |
| Mención de responsabilidad : |
Olga M Agudelo, Beatriz H Aristizabal, Stephanie K Yanow, Eliana Arango, Jaime Carmona-Fonseca, Amanda Maestre |
| Referencia : |
Malar J. 2014 Mar 27;13:122. |
| DOI (Digital Object Identifier) : |
10.1186/1475-2875-13-122 |
| PMID : |
24673747 |
| Derechos de uso : |
CC BY |
| En línea : |
https://malariajournal.biomedcentral.com/articles/10.1186/1475-2875-13-122 |
| Enlace permanente : |
https://hospitalpablotobon.cloudbiteca.com/pmb/opac_css/index.php?lvl=notice_dis |
|  |
Inicio
Clasificado(s) por (Año de edición descendente) Refinar búsquedaSubmicroscopic infection of placenta by Plasmodium produces Th1/Th2 cytokine imbalance, inflammation and hypoxia in women from north-west Colombia / Beatriz Helena Aristizábal Bernal
