Course of the experimental murine infection induced by sporothrix schenckii conidia according to depth of inoculation / Fabio Uribe Jaramillo ; Ángela Inés Zuluaga Cadena ; Ángela Restrepo Moreno  

Público ISBD Título : Course of the experimental murine infection induced by sporothrix schenckii conidia according to depth of inoculation Tipo de documento : documento electrónico Autores : Fabio Uribe Jaramillo, ; Ángela Inés Zuluaga Cadena, ; Ángela Restrepo Moreno, Fecha de publicación : 1993 Títulos uniformes : Journal of Medical and Veterinary Mycology Idioma : Inglés (eng) Resumen : Balb/c mice were infected intradermally (ID) or subcutaneously (SC) with Sporothrix schenckii conidia, in an attempt to demonstrate the role played by the depth of inoculation on the course of the infectious process. Animals were observed weekly up to 6 weeks and the time of appearance, as well as the aspect of the lesions, was recorded. Thirty percent of the animals were autopsied and their organs subjected to histopathological study. All animals, irrespective of the route of inoculation, developed clinically detectable sporotrichosis; the resulting lesions (nodules, drained or undrained abscesses, pustules) tended towards spontaneous healing; most of the ID-inoculated mice healed after 3 weeks post-infection while the SC group took longer (5 weeks). Histologically, all the animals that were killed had inflammatory foci, but a larger proportion (57%) of those infected SC exhibited granuloma formation in comparison with the animals infected by the ID route (16·6%). During the first week post-inoculation, the enlargement of the inguinal lymph node did not coincide with the presence of histologically detectable hypertrophy; however, after 2 weeks, 70–80% of all animals had both types of abnormality. Our results indicate that irrespective of the route of inoculation all the mice developed clinically and histologically detectable sporotrichosis, albeit of a limited character. Mención de responsabilidad : S. Restrepo-Gutierrez, M. Arango-Arteaga, F. Uribe-Jaramillo, A.I. Zuluaga-Cadena, A. Restrepo-Moreno DOI (Digital Object Identifier) : 10.1080/02681219380000541 En línea : https://academic.oup.com/mmy/article-abstract/31/6/411/988315 Enlace permanente : https://hospitalpablotobon.cloudbiteca.com/pmb/opac_css/index.php?lvl=notice_display&id=4371 Course of the experimental murine infection induced by sporothrix schenckii conidia according to depth of inoculation [documento electrónico] / Fabio Uribe Jaramillo, ; Ángela Inés Zuluaga Cadena, ; Ángela Restrepo Moreno,  . - 1993. Obra : Journal of Medical and Veterinary Mycology Idioma : Inglés (eng) Resumen : Balb/c mice were infected intradermally (ID) or subcutaneously (SC) with Sporothrix schenckii conidia, in an attempt to demonstrate the role played by the depth of inoculation on the course of the infectious process. Animals were observed weekly up to 6 weeks and the time of appearance, as well as the aspect of the lesions, was recorded. Thirty percent of the animals were autopsied and their organs subjected to histopathological study. All animals, irrespective of the route of inoculation, developed clinically detectable sporotrichosis; the resulting lesions (nodules, drained or undrained abscesses, pustules) tended towards spontaneous healing; most of the ID-inoculated mice healed after 3 weeks post-infection while the SC group took longer (5 weeks). Histologically, all the animals that were killed had inflammatory foci, but a larger proportion (57%) of those infected SC exhibited granuloma formation in comparison with the animals infected by the ID route (16·6%). During the first week post-inoculation, the enlargement of the inguinal lymph node did not coincide with the presence of histologically detectable hypertrophy; however, after 2 weeks, 70–80% of all animals had both types of abnormality. Our results indicate that irrespective of the route of inoculation all the mice developed clinically and histologically detectable sporotrichosis, albeit of a limited character. Mención de responsabilidad : S. Restrepo-Gutierrez, M. Arango-Arteaga, F. Uribe-Jaramillo, A.I. Zuluaga-Cadena, A. Restrepo-Moreno DOI (Digital Object Identifier) : 10.1080/02681219380000541 En línea : https://academic.oup.com/mmy/article-abstract/31/6/411/988315 Enlace permanente : https://hospitalpablotobon.cloudbiteca.com/pmb/opac_css/index.php?lvl=notice_display&id=4371

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Pathogenesis of paracoccidioidomycosis: A histopathological study of the experimental murine infection / Víctor Bedoya M. ; Juan Guillermo McEwen Ochoa ; Ángela María Tabares Velásquez ; Fabio Uribe Jaramillo ; Ángela Restrepo Moreno  

Público ISBD Título : Pathogenesis of paracoccidioidomycosis: A histopathological study of the experimental murine infection Tipo de documento : documento electrónico Autores : Víctor Bedoya M., ; Juan Guillermo McEwen Ochoa, ; Ángela María Tabares Velásquez, ; Fabio Uribe Jaramillo, ; Ángela Restrepo Moreno, Fecha de publicación : 1986 Títulos uniformes : Mycopathologia Idioma : Inglés (eng) Resumen : The pathogenesis of primary pulmonary P. brasiliensis infection, the systemic dissemination which followed, and the histopathology of the main organs involved was studied in a murine model of chronic paracoccidioidomycosis. Adult male BALB/C mice, were challenged intranasally with 26×10−6 viable P. brasiliensis yeast cells. We inoculated 86 animals which were sacrificed from 0 h to 20 weeks. As controls, 11 mice were instilled with saline solution, and 48 with 26×10−6 heat-killed. P. brasiliensis yeast cells. None of the animals receiving saline, exhibited pathologic alterations; 11.6% of those inoculated with the heatkilled cells, revealed mild, transitory acinopulmonary neutrophilic infiltrates. The animals infected with viable cells, developed a systemic process affecting mainly the lungs (46.5%), liver (18.6%), lymphnodes (18.6%), and spleen (3.5%). In this group of animals, lung lesions were detected regularly at all time periods from 3 h to 20 weeks. A multiple bronchoneumonic process was initially observed at 6 h, reached its maximum intensity around the third day, subsided thereafter but did not disappear and reactivated after the fifth week to become stationary until the end of experiments. Dissemination to other organs occurred early, and apparently by the hematogenous route. Initially the inflammatory cell infiltrate was mainly neutrophilic. With time, these cells were gradually replaced by lymphocytes, histiocytes and plasmocytes. Granuloma configuration of the cell infiltrate was distinctly seen around the fifth week, with multinucleated giant cells appearing at the ninth week. Hiliary lymphnode involvement was rare (7%) and primary lung lesions, as seen in tuberculosis and histoplasmosis, were not observed. Mención de responsabilidad : Víctor Bedoya, Juan G. McEwen, Angela M. Tabares, Fabio Uribe Jaramillo & Angela Restrepo Referencia : Mycopathologia. 1986 Jun;94(3):133-44. DOI (Digital Object Identifier) : 10.1007/BF00454591 PMID : 3762654 En línea : https://link.springer.com/article/10.1007%2FBF00454591 Enlace permanente : https://hospitalpablotobon.cloudbiteca.com/pmb/opac_css/index.php?lvl=notice_display&id=4338 Pathogenesis of paracoccidioidomycosis: A histopathological study of the experimental murine infection [documento electrónico] / Víctor Bedoya M., ; Juan Guillermo McEwen Ochoa, ; Ángela María Tabares Velásquez, ; Fabio Uribe Jaramillo, ; Ángela Restrepo Moreno,  . - 1986. Obra : Mycopathologia Idioma : Inglés (eng) Resumen : The pathogenesis of primary pulmonary P. brasiliensis infection, the systemic dissemination which followed, and the histopathology of the main organs involved was studied in a murine model of chronic paracoccidioidomycosis. Adult male BALB/C mice, were challenged intranasally with 26×10−6 viable P. brasiliensis yeast cells. We inoculated 86 animals which were sacrificed from 0 h to 20 weeks. As controls, 11 mice were instilled with saline solution, and 48 with 26×10−6 heat-killed. P. brasiliensis yeast cells. None of the animals receiving saline, exhibited pathologic alterations; 11.6% of those inoculated with the heatkilled cells, revealed mild, transitory acinopulmonary neutrophilic infiltrates. The animals infected with viable cells, developed a systemic process affecting mainly the lungs (46.5%), liver (18.6%), lymphnodes (18.6%), and spleen (3.5%). In this group of animals, lung lesions were detected regularly at all time periods from 3 h to 20 weeks. A multiple bronchoneumonic process was initially observed at 6 h, reached its maximum intensity around the third day, subsided thereafter but did not disappear and reactivated after the fifth week to become stationary until the end of experiments. Dissemination to other organs occurred early, and apparently by the hematogenous route. Initially the inflammatory cell infiltrate was mainly neutrophilic. With time, these cells were gradually replaced by lymphocytes, histiocytes and plasmocytes. Granuloma configuration of the cell infiltrate was distinctly seen around the fifth week, with multinucleated giant cells appearing at the ninth week. Hiliary lymphnode involvement was rare (7%) and primary lung lesions, as seen in tuberculosis and histoplasmosis, were not observed. Mención de responsabilidad : Víctor Bedoya, Juan G. McEwen, Angela M. Tabares, Fabio Uribe Jaramillo & Angela Restrepo Referencia : Mycopathologia. 1986 Jun;94(3):133-44. DOI (Digital Object Identifier) : 10.1007/BF00454591 PMID : 3762654 En línea : https://link.springer.com/article/10.1007%2FBF00454591 Enlace permanente : https://hospitalpablotobon.cloudbiteca.com/pmb/opac_css/index.php?lvl=notice_display&id=4338

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