Characterization of the use of the FilmArray Molecular panel for the diagnosis of central nervous system infection / Juan Pablo Villa Franco ; Santiago León Atehortúa Muñoz ; Zapata, Silvana ; Montoya Roldan, Daniel ; Isaza, Sandra Patricia ; Zapata, Carolina ; Lopez Estrada, Carolina ; Trompa, Iván  

Público ISBD Título : Characterization of the use of the FilmArray Molecular panel for the diagnosis of central nervous system infection Tipo de documento : documento electrónico Autores : Juan Pablo Villa Franco, Autor ; Santiago León Atehortúa Muñoz, Autor ; Zapata, Silvana, Autor ; Montoya Roldan, Daniel, Autor ; Isaza, Sandra Patricia, Autor ; Zapata, Carolina, Autor ; Lopez Estrada, Carolina, Autor ; Trompa, Iván, Autor Fecha de publicación : 2025 Títulos uniformes : Open Forum Infectious Diseases Idioma : Inglés (eng) Idioma original : Inglés (eng) Resumen : The Biofire FilmArray® Meningitis/Encephalitis Panel (FAME) has proven to be a valuable tool for etiological diagnosis. Despite this, we know little about its clinical impact.This investigation aims to characterize the clinical, epidemiological, therapeutic variables and outcomes of adults with suspected meningitis and/or encephalitis (ME) who underwent FAME panel, in two 3rd level hospitals in Medellín-Colombia. Mención de responsabilidad : Silvana zapata , Daniel Montoya Roldan, Infectious disease specialist , Pablo Villa franco, Infectious diseases MD , Sandra patricia isaza, Neurologist , Santiago Atehortua, Microbiology MSC MD , Carolina Zapata, MD , Carolina Lopez estrada, Internal medicine resident , Iván Trompa, infectious diseases MD Referencia : Open Forum Infectious Diseases, Volume 12, Issue Supplement_1, February 2025, ofae631.1114 DOI (Digital Object Identifier) : 10.1093/ofid/ofae631.1114 Derechos de uso : CC BY-NC-ND En línea : https://academic.oup.com/ofid/article/12/Supplement_1/ofae631.1114/7986720?login [...] Enlace permanente : https://hospitalpablotobon.cloudbiteca.com/pmb/opac_css/index.php?lvl=notice_dis Characterization of the use of the FilmArray Molecular panel for the diagnosis of central nervous system infection [documento electrónico] / Juan Pablo Villa Franco, Autor ; Santiago León Atehortúa Muñoz, Autor ; Zapata, Silvana, Autor ; Montoya Roldan, Daniel, Autor ; Isaza, Sandra Patricia, Autor ; Zapata, Carolina, Autor ; Lopez Estrada, Carolina, Autor ; Trompa, Iván, Autor . - 2025. Obra : Open Forum Infectious Diseases Idioma : Inglés (eng) Idioma original : Inglés (eng) Resumen : The Biofire FilmArray® Meningitis/Encephalitis Panel (FAME) has proven to be a valuable tool for etiological diagnosis. Despite this, we know little about its clinical impact.This investigation aims to characterize the clinical, epidemiological, therapeutic variables and outcomes of adults with suspected meningitis and/or encephalitis (ME) who underwent FAME panel, in two 3rd level hospitals in Medellín-Colombia. Mención de responsabilidad : Silvana zapata , Daniel Montoya Roldan, Infectious disease specialist , Pablo Villa franco, Infectious diseases MD , Sandra patricia isaza, Neurologist , Santiago Atehortua, Microbiology MSC MD , Carolina Zapata, MD , Carolina Lopez estrada, Internal medicine resident , Iván Trompa, infectious diseases MD Referencia : Open Forum Infectious Diseases, Volume 12, Issue Supplement_1, February 2025, ofae631.1114 DOI (Digital Object Identifier) : 10.1093/ofid/ofae631.1114 Derechos de uso : CC BY-NC-ND En línea : https://academic.oup.com/ofid/article/12/Supplement_1/ofae631.1114/7986720?login [...] Enlace permanente : https://hospitalpablotobon.cloudbiteca.com/pmb/opac_css/index.php?lvl=notice_dis

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Biofire FilmArray Meningitis/Encephalitis panel for the aetiological diagnosis of central nervous system infections: A systematic review and diagnostic test accuracy meta-analysis / Santiago León Atehortúa Muñoz    

Público ISBD Título : Biofire FilmArray Meningitis/Encephalitis panel for the aetiological diagnosis of central nervous system infections: A systematic review and diagnostic test accuracy meta-analysis Tipo de documento : documento electrónico Autores : Santiago León Atehortúa Muñoz, Fecha de publicación : 2022 Títulos uniformes : eClinicalMedicine Idioma : Inglés (eng) Palabras clave : CNS infection  Diagnostic accuracy  Encephalitis  Film array  Meningitis  Meta-analysis  Multiplex PCR Resumen : Background: The FilmArray Meningitis/Encephalitis(FA/ME) panel brings benefits in clinical practice, but its diagnostic test accuracy (DTA) remains unclear. We aimed to determine the DTA of FA/ME for the aetiological diagnostic in patients with suspected central nervous system(CNS) infection. Methods: We performed a systematic review with DTA meta-analysis (PROSPERO: CRD42020139285). We searched Embase, Medline (Ovid), and Web of Science from inception until September 1st, 2021. We assessed the study-level risk of bias with the QUADAS-2 tool and applied the GRADE approach to assess the certainty of the synthesised evidence. We included studies that simultaneously measured the reference test (CSF/blood culture for bacteria, and specific polymerase chain reaction for viruses) and the FA/ME in patients with suspected CNS infection. We performed random-effects bivariate meta-analysis models of combined sensitivity and specificity using CSF/blood cultures(reference test 1) and a final diagnosis adjudication based on clinical/laboratory criteria (reference test 2). Findings: We included 19 studies (11,351 participants). For all bacteria with reference test 1 (16 studies/6183 patients) sensitivity was estimated at 89·5% (95%CI 81·1-94·4), and specificity at 97·4% (95%CI 94-98·9). With reference test 2 (15 studies/5,524 patients), sensitivity was estimated at 92·1%(95%CI 86·8-95·3) and specificity at 99.2(95%CI 98·3-99·6) For herpes simplex virus-2(HSV-2), enteroviruses, and Varicella-Zoster virus (VZV), we obtained sensitivities between 75·5 and 93·8%, and specificities above 99% (reference test 1). Certainty of the evidence was low. Interpretation: FA/ME may have acceptable-to-high sensitivities and high specificities for identifying bacteria, especially for S.pneumoniae, and viruses, especially for HSV-2, and enteroviruses. Sensitivities for L.monocytogenes, H.influenzae, E.coli, and HSV-1 were suboptimal. Mención de responsabilidad : Juliana Trujillo-Gomez, Sofia Tsokani, Catalina Arango-Ferreira, Santiago Atehortua-Muñoz, Maria José Jimenez-Villegas, Carolina Serrano-Tabares, Areti-Angeliki Veroniki, and Ivan D. Florez Referencia : EClinicalMedicine. 2022 Feb 14;44:101275. DOI (Digital Object Identifier) : 10.1016/j.eclinm.2022.101275 PMID : 35198914 Derechos de uso : CC BY-NC-ND En línea : https://linkinghub.elsevier.com/retrieve/pii/S2589537022000050 Enlace permanente : https://hospitalpablotobon.cloudbiteca.com/pmb/opac_css/index.php?lvl=notice_dis Biofire FilmArray Meningitis/Encephalitis panel for the aetiological diagnosis of central nervous system infections: A systematic review and diagnostic test accuracy meta-analysis [documento electrónico] / Santiago León Atehortúa Muñoz,  . - 2022. Obra : eClinicalMedicine Idioma : Inglés (eng) Palabras clave : CNS infection  Diagnostic accuracy  Encephalitis  Film array  Meningitis  Meta-analysis  Multiplex PCR Resumen : Background: The FilmArray Meningitis/Encephalitis(FA/ME) panel brings benefits in clinical practice, but its diagnostic test accuracy (DTA) remains unclear. We aimed to determine the DTA of FA/ME for the aetiological diagnostic in patients with suspected central nervous system(CNS) infection. Methods: We performed a systematic review with DTA meta-analysis (PROSPERO: CRD42020139285). We searched Embase, Medline (Ovid), and Web of Science from inception until September 1st, 2021. We assessed the study-level risk of bias with the QUADAS-2 tool and applied the GRADE approach to assess the certainty of the synthesised evidence. We included studies that simultaneously measured the reference test (CSF/blood culture for bacteria, and specific polymerase chain reaction for viruses) and the FA/ME in patients with suspected CNS infection. We performed random-effects bivariate meta-analysis models of combined sensitivity and specificity using CSF/blood cultures(reference test 1) and a final diagnosis adjudication based on clinical/laboratory criteria (reference test 2). Findings: We included 19 studies (11,351 participants). For all bacteria with reference test 1 (16 studies/6183 patients) sensitivity was estimated at 89·5% (95%CI 81·1-94·4), and specificity at 97·4% (95%CI 94-98·9). With reference test 2 (15 studies/5,524 patients), sensitivity was estimated at 92·1%(95%CI 86·8-95·3) and specificity at 99.2(95%CI 98·3-99·6) For herpes simplex virus-2(HSV-2), enteroviruses, and Varicella-Zoster virus (VZV), we obtained sensitivities between 75·5 and 93·8%, and specificities above 99% (reference test 1). Certainty of the evidence was low. Interpretation: FA/ME may have acceptable-to-high sensitivities and high specificities for identifying bacteria, especially for S.pneumoniae, and viruses, especially for HSV-2, and enteroviruses. Sensitivities for L.monocytogenes, H.influenzae, E.coli, and HSV-1 were suboptimal. Mención de responsabilidad : Juliana Trujillo-Gomez, Sofia Tsokani, Catalina Arango-Ferreira, Santiago Atehortua-Muñoz, Maria José Jimenez-Villegas, Carolina Serrano-Tabares, Areti-Angeliki Veroniki, and Ivan D. Florez Referencia : EClinicalMedicine. 2022 Feb 14;44:101275. DOI (Digital Object Identifier) : 10.1016/j.eclinm.2022.101275 PMID : 35198914 Derechos de uso : CC BY-NC-ND En línea : https://linkinghub.elsevier.com/retrieve/pii/S2589537022000050 Enlace permanente : https://hospitalpablotobon.cloudbiteca.com/pmb/opac_css/index.php?lvl=notice_dis

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