Título : |
Survival of paracoccidioides brasiliensis yeast cells under microaerophilic conditions |
Tipo de documento : |
documento electrónico |
Autores : |
Ángela Restrepo Moreno, ; Beatriz Elena Jiménez, ; Catalina de Bedout Gómez, |
Fecha de publicación : |
1981 |
Títulos uniformes : |
Journal of Medical and Veterinary Mycology
|
Idioma : |
Inglés (eng) |
Resumen : |
The ability of P. brasiliensis yeast cells to withstand microaerophilic conditions was investigated in a liquid medium distributed in tall columns in screw-capped tubes. Young cells of three isolates were inoculated on top of the medium, and the tubes were incubated aerobically and anaerobically at 36°C for 28 days. The viability of cells that had sedimented to the bottoms of the tubes was studied by fluorescent microscopy and by their capacity to resume growth when transferred to fresh medium under continuous agitation. The proportion of viable cells in the sediments diminished with time of incubation. However, after 28 days, 27% of the cells were still viable and fully capable of active growth when placed under adequate aeration. On the other hand, drastic reduction of oxygen access elicited an accelerated death rate, with no survival after 7 days of incubation. |
Mención de responsabilidad : |
Angela Restrepo, Beatriz Elena Jiménez, Catalina de Bedout |
Referencia : |
Sabouraudia. 1981 Dec;19(4):301-5. |
DOI (Digital Object Identifier) : |
10.1080/00362178185380481 |
PMID : |
7034241 |
En línea : |
https://academic.oup.com/mmy/article-abstract/19/4/301/1697303 |
Enlace permanente : |
https://hospitalpablotobon.cloudbiteca.com/pmb/opac_css/index.php?lvl=notice_display&id=4320 |
Survival of paracoccidioides brasiliensis yeast cells under microaerophilic conditions [documento electrónico] / Ángela Restrepo Moreno, ; Beatriz Elena Jiménez, ; Catalina de Bedout Gómez, . - 1981. Obra : Journal of Medical and Veterinary MycologyIdioma : Inglés ( eng) Resumen : |
The ability of P. brasiliensis yeast cells to withstand microaerophilic conditions was investigated in a liquid medium distributed in tall columns in screw-capped tubes. Young cells of three isolates were inoculated on top of the medium, and the tubes were incubated aerobically and anaerobically at 36°C for 28 days. The viability of cells that had sedimented to the bottoms of the tubes was studied by fluorescent microscopy and by their capacity to resume growth when transferred to fresh medium under continuous agitation. The proportion of viable cells in the sediments diminished with time of incubation. However, after 28 days, 27% of the cells were still viable and fully capable of active growth when placed under adequate aeration. On the other hand, drastic reduction of oxygen access elicited an accelerated death rate, with no survival after 7 days of incubation. |
Mención de responsabilidad : |
Angela Restrepo, Beatriz Elena Jiménez, Catalina de Bedout |
Referencia : |
Sabouraudia. 1981 Dec;19(4):301-5. |
DOI (Digital Object Identifier) : |
10.1080/00362178185380481 |
PMID : |
7034241 |
En línea : |
https://academic.oup.com/mmy/article-abstract/19/4/301/1697303 |
Enlace permanente : |
https://hospitalpablotobon.cloudbiteca.com/pmb/opac_css/index.php?lvl=notice_display&id=4320 |
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