Morphological study of a variant of Paracoccidioides brasiliensis that exists in the yeast form at room temperature [documento electrónico] / L. A. Villar, ; Maria E. Salazar, ; Ángela Restrepo Moreno,  . - 1988.
Obra : Journal of Medical and Veterinary Mycology

Idioma : Inglés (eng)

Resumen :	Incubation of Paracoccidioides brasiliensis conidia at 20–25°C (RT) results in two types of colonies, mycelial (M) and yeast (YRT). A study of the latter colonies was undertaken. Conidia were plated in complex (BHI) and chemically-defined media (CDCM), with and without fetal calf serum (FCS). Incubation was carried out at 21°C for 4 weeks. The mean number of YRT colonies was approximately 18·5% on BHI agar. Selected YRT colonies were transferred to liquid CDCM, incubated 7 days at 21°C and transferred to solid media; YRT appearance was preserved in 95% of the colonies if media were FCS-supplemented; otherwise, most colonies reverted to the M form. When FCS was replaced by bovine albumin or by alpha-globulin, 63% and 68% respectively of the colonies obtained after plating YRT cells, became mycelial. Comparative morphologic studies of both YRT and yeasts grown at 37°C suggested that there were no major differences between these two types of yeast cells when size and budding were taken into consideration. The results indicate that in this particular variant, dimorphism is not exclusively temperature-dependent.
Mención de responsabilidad :	L.A. Villar, M.E. Salazar, A. Restrepo
Referencia :	J Med Vet Mycol. 1988;26(5):269-76
DOI (Digital Object Identifier) :	10.1080/02681218880000381
PMID :	3236146
En línea :	https://academic.oup.com/mmy/article-abstract/26/5/269/895907
Enlace permanente :	https://hospitalpablotobon.cloudbiteca.com/pmb/opac_css/index.php?lvl=notice_display&id=4347