A culture medium for paracoccidioides brasiliensis with high plating efficiency, and the effect of siderophores [documento electrónico] / Juan Guillermo McEwen Ochoa,  . - 1988.
Obra : Journal of Medical and Veterinary Mycology

Idioma : Inglés (eng)

Resumen :	The plating efficiency of Paracoccidioides brasiliensis on standard mycological media is poor, impairing its isolation and recovery from various sources, particularly infected tissues. We describe a medium that markedly improves P. brasiliensis plating efficiency. It consists of a synthetic medium (modified McVeigh-Morton) supplemented with 4% (v:v) horse serum and 5% (v:v) culture filtrate from stationary phase P. brasiliensis cultures. A commercially available medium (brain-heart infusion), ordinarily inferior to unsupplemented McVeigh-Morton medium, is at least as efficacious as supplemented McVeigh-Morton medium when supplemented in this manner. We show that plating efficiency varies among P. brasiliensis isolates and can even vary with the isolate's history of passage in culture. In contrast, all isolates studied could produce the growth enhancing factors present in culture filtrate, whereas others can be substituted for both the filtrate and serum. The enhancing effect of filtrate and/or serum could be removed by chelating iron. P. brasiliensis-produced siderophores are likely to be the growth enhancing moiety in culture filtrates.
Mención de responsabilidad :	E. Castaneda, E. Brummer, A.M. Perlman, J.G. McEwen, D.A. Stevens
Referencia :	J Med Vet Mycol. 1988;26(6):351-8.
DOI (Digital Object Identifier) :	10.1080/02681218880000501
PMID :	2977619
En línea :	https://academic.oup.com/mmy/article-abstract/26/6/351/917657
Enlace permanente :	https://hospitalpablotobon.cloudbiteca.com/pmb/opac_css/index.php?lvl=notice_display&id=4350