Virulence of Paracoccidioides brasiliensis: The influence of in vitro passage and storage [documento electrónico] / Ángela Restrepo Moreno,  . - 1990.
Obra : Mycopathologia

Idioma : Inglés (eng)

Palabras clave :	Paracoccidioides brasiliensis  Paracoccidioidomycosis  passage in vitro  virulence
Resumen :	Stability of virulence in P. brasiliensis isolates was studied with respect to the in vitro culture history and methods used for storage. Virulence in yeast-form P. brasiliensis isolates was tested in a chronic pulmonary murine model of paracoccidioidomycosis where progression of disease was quantitated in terms of colony forming units recoverable from lungs. Four isolates of P. brasiliensis, including recently isolated from patients or experimental animals, caused chronic progressive disease. Two isolates with a history of subculturing showed attenuation by causing resolving but chronic disease. An attenuated isolate became avirulent subsequent to 15 more years of subculturing. These findings suggest that virulence of P. brasiliensis can be attenuated or lost subsequent to cycles of subculturing over long periods. Our data suggest that the use of fresh P. brasiliensis isolates may be needed to provide reproducible virulence for experimental systems.
Mención de responsabilidad :	Elmer Brummer, Angela Restrepo, Linda H. Hanson & David A. Stevens
Referencia :	Mycopathologia. 1990 Jan;109(1):13-7.
DOI (Digital Object Identifier) :	10.1007/BF00437001
PMID :	2139178
En línea :	https://link.springer.com/article/10.1007%2FBF00437001
Enlace permanente :	https://hospitalpablotobon.cloudbiteca.com/pmb/opac_css/index.php?lvl=notice_display&id=4358