Lysozyme plays a dual role against the dimorphic fungus Paracoccidioides brasiliensis [documento electrónico] / Beatriz Helena Aristizábal Bernal,  . - 2008.
Obra : Revista do Instituto de Medicina Tropical de São Paulo

Idioma : Inglés (eng)

Palabras clave :	Lysozyme  TNF-  Alveolar macrophages  Paracoccidioides brasiliensis
Resumen :	In order to determine the role of lysozyme, an antimicrobial peptide belonging to the innate immune system, against the dimorphic fungus Paracoccidioides brasiliensis, co-cultures of the MH-S murine alveolar macrophages cell line with P. brasiliensis conidia were done; assays to evaluate the effect of physiological and inflammatory concentrations of lysozyme directly on the fungus life cycle were also undertaken. We observed that TNF-α-activated macrophages significantly inhibited the conidia to yeast transition (p = 0.0043) and exerted an important fungicidal effect (p = 0.0044), killing 27% more fungal propagules in comparison with controls. Nonetheless, after adding a selective inhibitor of lysozyme, the fungicidal effect was reverted. When P. brasiliensis propagules were exposed directly to different concentrations of lysozyme, a dual effect was observed. Physiologic concentrations of the enzyme facilitated the conidia-to-yeast transition process (p
Mención de responsabilidad :	Damaris Lopera; Beatriz H. Aristizabal; Angela Restrepo; Luz Elena Cano; Ángel González
Referencia :	Rev Inst Med Trop Sao Paulo. May-Jun 2008;50(3):169-75.
DOI (Digital Object Identifier) :	10.1590/S0036-46652008000300008
PMID :	18604417
Derechos de uso :	CC BY-NC
En línea :	http://www.scielo.br/scielo.php?script=sci_abstract&pid=S0036-46652008000300008
Enlace permanente :	https://hospitalpablotobon.cloudbiteca.com/pmb/opac_css/index.php?lvl=notice_display&id=4438