Flow cytometry assessment of graft-infiltrating lymphocytes can accurately identify acute rejection in kidney transplants [documento electrónico] / Gloria Lucía Lema Zuluaga,  . - 2014.
Obra : Clinical Transplantation

Idioma : Inglés (eng)

Palabras clave :	aspiration biopsy: flow cytometry  kidney transplants  peripheral blood lymphocytes
Resumen :	Background: Previously, we have reported that flow cytometry analysis of fine‐needle aspirates can accurately predict rejection in kidney transplants treated with cyclosporine–azathioprine–prednisolone. In this study, we examined this technique's accuracy using current immunosuppression. Methods: Kidney transplant recipients were treated with calcineurin inhibitors, mycophenolate mofetil, and prednisolone: 92 remained rejection‐free – Group I – and 37 developed acute rejection – Group II. An allograft aspiration specimen and peripheral blood were collected from Group I on post‐transplant day 7 and from Group II on the day of clinical rejection. Results: Significant changes were seen in both aspiration and peripheral blood samples in several T cell subsets when comparing Groups I and II. A sensitivity of 94.6%, specificity of 85%, and AUC = 0.966 were observed through combining CD8DR with CD3CD69 values from aspiration specimen; the corresponding AUC in peripheral blood was 0.847. Irreversible rejections displayed a significantly higher activation score (p = 0.024). Conclusions: Flow cytometry analysis of aspiration specimen achieved high diagnostic performance in renal transplants through studying CD8DR and CD3CD69 under current immunosuppressive therapy. Peripheral blood analysis, although not significant, showed the same trend. The activation score anticipated the irreversibility of rejection. The data suggest this test, through an easily tolerated technique, merits further diagnostic use.
Mención de responsabilidad :	Paula D P Xavier, Gloria L Lema, Maria C Magalhães, Armando Teixeira-Pinto, Susana Sampaio-Norton, Sérgio Gaião, Tiago Guimarães, José G G Oliveira
Referencia :	Clin Transplant. 2014 Feb;28(2):177-83.
DOI (Digital Object Identifier) :	10.1111/ctr.12293
PMID :	24372696
En línea :	https://onlinelibrary.wiley.com/doi/abs/10.1111/ctr.12293
Enlace permanente :	https://hospitalpablotobon.cloudbiteca.com/pmb/opac_css/index.php?lvl=notice_display&id=4557