Virulence of a variant of Paracoccidioides brasiliensis that exists in the yeast form at room temperature / L. A. Villar ; Ángela Restrepo Moreno  

Público ISBD Título : Virulence of a variant of Paracoccidioides brasiliensis that exists in the yeast form at room temperature Tipo de documento : documento electrónico Autores : L. A. Villar, ; Ángela Restrepo Moreno, Fecha de publicación : 1989 Títulos uniformes : Journal of Medical and Veterinary Mycology Idioma : Inglés (eng) Resumen : Paracoccidioides brasiliensis (ATCC 60885) produces conidia that, when plated in enriched media and incubated at 21-25 degrees C, give rise to a yeast-form variant (YRT) (ATCC 46678). The virulence of this variant for BALB/c mice has been compared with that of the yeast and conidia produced by the parent isolate. Adult female mice were inoculated intravenously with 10(7) viable cells of YRT or yeast, or 5 x 10(5) viable conidia. Mice were weighed weekly, and three mice were sacrificed at 1, 2, 3, 4 and 8 weeks post-inoculation and their organs weighed and prepared for determination of viable counts (colony forming units; c.f.u.). Animals infected with yeast cells or with conidia exhibited little weight loss in comparison with that seen in the YRT-infected mice which began to lose weight 1 week post-challenge. Organ weights increased in the YRT group, while the changes in the remaining two groups were minor. The c.f.u. from these organs also increased in the YRT-infected mice, whereas the infection in the yeast-and conidia-infected mice was self-limiting. A proportion (16.6%) of the YRT-infected animals died during the observation period. Conversely, no deaths occurred in the remaining groups. These results indicate that the YRT variant possesses increased virulence. Mención de responsabilidad : L.A. Villar, A. Restrepo Referencia : J Med Vet Mycol. 1989;27(3):141-8 DOI (Digital Object Identifier) : 10.1080/02681218980000201 PMID : 2778575 En línea : https://academic.oup.com/mmy/article-abstract/27/3/141/896291 Enlace permanente : https://hospitalpablotobon.cloudbiteca.com/pmb/opac_css/index.php?lvl=notice_display&id=4353 Virulence of a variant of Paracoccidioides brasiliensis that exists in the yeast form at room temperature [documento electrónico] / L. A. Villar, ; Ángela Restrepo Moreno,  . - 1989. Obra : Journal of Medical and Veterinary Mycology Idioma : Inglés (eng) Resumen : Paracoccidioides brasiliensis (ATCC 60885) produces conidia that, when plated in enriched media and incubated at 21-25 degrees C, give rise to a yeast-form variant (YRT) (ATCC 46678). The virulence of this variant for BALB/c mice has been compared with that of the yeast and conidia produced by the parent isolate. Adult female mice were inoculated intravenously with 10(7) viable cells of YRT or yeast, or 5 x 10(5) viable conidia. Mice were weighed weekly, and three mice were sacrificed at 1, 2, 3, 4 and 8 weeks post-inoculation and their organs weighed and prepared for determination of viable counts (colony forming units; c.f.u.). Animals infected with yeast cells or with conidia exhibited little weight loss in comparison with that seen in the YRT-infected mice which began to lose weight 1 week post-challenge. Organ weights increased in the YRT group, while the changes in the remaining two groups were minor. The c.f.u. from these organs also increased in the YRT-infected mice, whereas the infection in the yeast-and conidia-infected mice was self-limiting. A proportion (16.6%) of the YRT-infected animals died during the observation period. Conversely, no deaths occurred in the remaining groups. These results indicate that the YRT variant possesses increased virulence. Mención de responsabilidad : L.A. Villar, A. Restrepo Referencia : J Med Vet Mycol. 1989;27(3):141-8 DOI (Digital Object Identifier) : 10.1080/02681218980000201 PMID : 2778575 En línea : https://academic.oup.com/mmy/article-abstract/27/3/141/896291 Enlace permanente : https://hospitalpablotobon.cloudbiteca.com/pmb/opac_css/index.php?lvl=notice_display&id=4353

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Morphological study of a variant of Paracoccidioides brasiliensis that exists in the yeast form at room temperature / L. A. Villar ; Maria E. Salazar ; Ángela Restrepo Moreno  

Público ISBD Título : Morphological study of a variant of Paracoccidioides brasiliensis that exists in the yeast form at room temperature Tipo de documento : documento electrónico Autores : L. A. Villar, ; Maria E. Salazar, ; Ángela Restrepo Moreno, Fecha de publicación : 1988 Títulos uniformes : Journal of Medical and Veterinary Mycology Idioma : Inglés (eng) Resumen : Incubation of Paracoccidioides brasiliensis conidia at 20–25°C (RT) results in two types of colonies, mycelial (M) and yeast (YRT). A study of the latter colonies was undertaken. Conidia were plated in complex (BHI) and chemically-defined media (CDCM), with and without fetal calf serum (FCS). Incubation was carried out at 21°C for 4 weeks. The mean number of YRT colonies was approximately 18·5% on BHI agar. Selected YRT colonies were transferred to liquid CDCM, incubated 7 days at 21°C and transferred to solid media; YRT appearance was preserved in 95% of the colonies if media were FCS-supplemented; otherwise, most colonies reverted to the M form. When FCS was replaced by bovine albumin or by alpha-globulin, 63% and 68% respectively of the colonies obtained after plating YRT cells, became mycelial. Comparative morphologic studies of both YRT and yeasts grown at 37°C suggested that there were no major differences between these two types of yeast cells when size and budding were taken into consideration. The results indicate that in this particular variant, dimorphism is not exclusively temperature-dependent. Mención de responsabilidad : L.A. Villar, M.E. Salazar, A. Restrepo Referencia : J Med Vet Mycol. 1988;26(5):269-76 DOI (Digital Object Identifier) : 10.1080/02681218880000381 PMID : 3236146 En línea : https://academic.oup.com/mmy/article-abstract/26/5/269/895907 Enlace permanente : https://hospitalpablotobon.cloudbiteca.com/pmb/opac_css/index.php?lvl=notice_display&id=4347 Morphological study of a variant of Paracoccidioides brasiliensis that exists in the yeast form at room temperature [documento electrónico] / L. A. Villar, ; Maria E. Salazar, ; Ángela Restrepo Moreno,  . - 1988. Obra : Journal of Medical and Veterinary Mycology Idioma : Inglés (eng) Resumen : Incubation of Paracoccidioides brasiliensis conidia at 20–25°C (RT) results in two types of colonies, mycelial (M) and yeast (YRT). A study of the latter colonies was undertaken. Conidia were plated in complex (BHI) and chemically-defined media (CDCM), with and without fetal calf serum (FCS). Incubation was carried out at 21°C for 4 weeks. The mean number of YRT colonies was approximately 18·5% on BHI agar. Selected YRT colonies were transferred to liquid CDCM, incubated 7 days at 21°C and transferred to solid media; YRT appearance was preserved in 95% of the colonies if media were FCS-supplemented; otherwise, most colonies reverted to the M form. When FCS was replaced by bovine albumin or by alpha-globulin, 63% and 68% respectively of the colonies obtained after plating YRT cells, became mycelial. Comparative morphologic studies of both YRT and yeasts grown at 37°C suggested that there were no major differences between these two types of yeast cells when size and budding were taken into consideration. The results indicate that in this particular variant, dimorphism is not exclusively temperature-dependent. Mención de responsabilidad : L.A. Villar, M.E. Salazar, A. Restrepo Referencia : J Med Vet Mycol. 1988;26(5):269-76 DOI (Digital Object Identifier) : 10.1080/02681218880000381 PMID : 3236146 En línea : https://academic.oup.com/mmy/article-abstract/26/5/269/895907 Enlace permanente : https://hospitalpablotobon.cloudbiteca.com/pmb/opac_css/index.php?lvl=notice_display&id=4347

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