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Autor Maria E. Salazar |
Documentos disponibles escritos por este autor (8)
Clasificado(s) por (Año de edición descendente) Refinar búsquedaKilling of Paracoccidioides brasiliensis conidia by pulmonary macrophages and the effect of cytokines / Luz Elena Cano Restrepo ; R. Arango ; Maria E. Salazar ; Ángela Restrepo Moreno
Título : Killing of Paracoccidioides brasiliensis conidia by pulmonary macrophages and the effect of cytokines Tipo de documento : documento electrónico Autores : Luz Elena Cano Restrepo, ; R. Arango, ; Maria E. Salazar, ; Ángela Restrepo Moreno, Fecha de publicación : 1992 Títulos uniformes : Journal of Medical and Veterinary Mycology Idioma : Inglés (eng) Resumen : The ability of conidia, the infectious form of the dimorphic fungus Paracoccidioides brasiliensis, to be killed in vitro by murine pulmonary macrophages was studied. Mice were immunized by intravenous injection of killed conidia, which resulted in cellular immunity demonstrated by delayed type hypersensitivity in vivo and macrophage migration inhibition factor production in vitro. Resident pulmonary macrophages from non-immune mice were able to significantly kill the conidia (28%). Such macrophages treated with supernatants (cytokines) from antigen-stimulated immune mononuclears had a markedly enhanced ability to kill conidia (73%). These results show that activated pulmonary macrophages are potent killers of conidia of P. brasiliensis and that immune mononuclears play a role in activation of macrophages. Activated macrophages may be important for pulmonary defense against the initial stages of infection with this fungus. Mención de responsabilidad : L.E. Cano, R. Arango, M.E. Salazar, E. Brummer, D.A. Stevens & A. Restrepo Referencia : J Med Vet Mycol. 1992;30(2):161-8. DOI (Digital Object Identifier) : 10.1080/02681219280000211 PMID : 1588466 En línea : https://www.tandfonline.com/doi/abs/10.1080/02681219280000211 Enlace permanente : https://hospitalpablotobon.cloudbiteca.com/pmb/opac_css/index.php?lvl=notice_display&id=4365 Killing of Paracoccidioides brasiliensis conidia by pulmonary macrophages and the effect of cytokines [documento electrónico] / Luz Elena Cano Restrepo, ; R. Arango, ; Maria E. Salazar, ; Ángela Restrepo Moreno, . - 1992.
Obra : Journal of Medical and Veterinary Mycology
Idioma : Inglés (eng)
Resumen : The ability of conidia, the infectious form of the dimorphic fungus Paracoccidioides brasiliensis, to be killed in vitro by murine pulmonary macrophages was studied. Mice were immunized by intravenous injection of killed conidia, which resulted in cellular immunity demonstrated by delayed type hypersensitivity in vivo and macrophage migration inhibition factor production in vitro. Resident pulmonary macrophages from non-immune mice were able to significantly kill the conidia (28%). Such macrophages treated with supernatants (cytokines) from antigen-stimulated immune mononuclears had a markedly enhanced ability to kill conidia (73%). These results show that activated pulmonary macrophages are potent killers of conidia of P. brasiliensis and that immune mononuclears play a role in activation of macrophages. Activated macrophages may be important for pulmonary defense against the initial stages of infection with this fungus. Mención de responsabilidad : L.E. Cano, R. Arango, M.E. Salazar, E. Brummer, D.A. Stevens & A. Restrepo Referencia : J Med Vet Mycol. 1992;30(2):161-8. DOI (Digital Object Identifier) : 10.1080/02681219280000211 PMID : 1588466 En línea : https://www.tandfonline.com/doi/abs/10.1080/02681219280000211 Enlace permanente : https://hospitalpablotobon.cloudbiteca.com/pmb/opac_css/index.php?lvl=notice_display&id=4365 Reserva
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Código de barras Número de Ubicación Tipo de medio Ubicación Sección Estado DD000865 AC-1992-004 Archivo digital Producción Científica Artículos científicos Disponible
Título : Electron microscopic study of conidia produced by the mycelium of Paracoccidioides brasiliensis Tipo de documento : documento electrónico Autores : Maria E. Salazar, ; Luz Elena Cano Restrepo, ; Ángela Restrepo Moreno, Fecha de publicación : 1991 Títulos uniformes : Mycopathologia Idioma : Inglés (eng) Palabras clave : Paracoccidioidomycosis mycelium conidia ultrastructure Resumen : The ultrastructure of asexual spores (conidia) produced by the mycelial form of Paracoccidioides brasiliensis was studied for the first time with transmission electron microscopy, using thin sections of aldehyde-osmium-fixed and epoxy-resin-embedded samples. The various types of conidia observed in the sections correlated well with previous light-microscopic descriptions. These types were intercalary or apical conidia, depending on their location along the originating hyphae. As in previous studies they were characterized as arthroconidia, aleuriospores and sessile or pedunculate pyriform conidia. The sporogenous cells were clearly distinguished from hyphal cells by the thickness and appearance of their cell walls. Copious fibrillar material (glycocalyx) detected at the cell surface was stained with ruthenium red during the fixation process. Typical subcellular organelles (nucleus, nucleolus, mitochondria, ribosomes, etc) were found in most of the sections. It was concluded that the spores produced by the mycelial phase of P. brasiliensis possess all attributes of viable and physiologically competent eukaryotic cells. Mención de responsabilidad : Mercedes R. Edwards, M. E. Salazar, W. A. Samsonoff, L. E. Cano, G. A. Ostrander & A. Restrepo Referencia : Mycopathologia. 1991 Jun;114(3):169-77. DOI (Digital Object Identifier) : 10.1007/BF00437210 PMID : 1886643 En línea : https://link.springer.com/article/10.1007/BF00437210 Enlace permanente : https://hospitalpablotobon.cloudbiteca.com/pmb/opac_css/index.php?lvl=notice_display&id=4360 Electron microscopic study of conidia produced by the mycelium of Paracoccidioides brasiliensis [documento electrónico] / Maria E. Salazar, ; Luz Elena Cano Restrepo, ; Ángela Restrepo Moreno, . - 1991.
Obra : Mycopathologia
Idioma : Inglés (eng)
Palabras clave : Paracoccidioidomycosis mycelium conidia ultrastructure Resumen : The ultrastructure of asexual spores (conidia) produced by the mycelial form of Paracoccidioides brasiliensis was studied for the first time with transmission electron microscopy, using thin sections of aldehyde-osmium-fixed and epoxy-resin-embedded samples. The various types of conidia observed in the sections correlated well with previous light-microscopic descriptions. These types were intercalary or apical conidia, depending on their location along the originating hyphae. As in previous studies they were characterized as arthroconidia, aleuriospores and sessile or pedunculate pyriform conidia. The sporogenous cells were clearly distinguished from hyphal cells by the thickness and appearance of their cell walls. Copious fibrillar material (glycocalyx) detected at the cell surface was stained with ruthenium red during the fixation process. Typical subcellular organelles (nucleus, nucleolus, mitochondria, ribosomes, etc) were found in most of the sections. It was concluded that the spores produced by the mycelial phase of P. brasiliensis possess all attributes of viable and physiologically competent eukaryotic cells. Mención de responsabilidad : Mercedes R. Edwards, M. E. Salazar, W. A. Samsonoff, L. E. Cano, G. A. Ostrander & A. Restrepo Referencia : Mycopathologia. 1991 Jun;114(3):169-77. DOI (Digital Object Identifier) : 10.1007/BF00437210 PMID : 1886643 En línea : https://link.springer.com/article/10.1007/BF00437210 Enlace permanente : https://hospitalpablotobon.cloudbiteca.com/pmb/opac_css/index.php?lvl=notice_display&id=4360 Reserva
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Código de barras Número de Ubicación Tipo de medio Ubicación Sección Estado DD000860 AC-1991-002 Archivo digital Producción Científica Artículos científicos Disponible
Título : Scanning electron microscopy of the conidia produced by the mycelial form of Paracoccidioides brasiliensis Tipo de documento : documento electrónico Autores : Maria E. Salazar, ; Ángela Restrepo Moreno, ; Luz Elena Cano Restrepo, Fecha de publicación : 1991 Títulos uniformes : Mycopathologia Idioma : Inglés (eng) Palabras clave : Mycelial conidia Paracoccidioides brasiliensis scanning electron microscopy Resumen : The conidia produced by the mycelial form of Paracoccidioides brasiliensis were examined by scanning electron microscopy for the first time. Several different conidial types were characterized. These included intercalary arthroconidia, several types of septate conidia that are formed from other conidia, pedunculate conidia, and terminal hyphal conidia. In addition, the ultrastructure of the supporting pedestal of the pedunculate conidium was found to be separated from the mother conidium by a septum in some instances, and at other times it was not. Mención de responsabilidad : William A. Samsonoff, M. E. Salazar, M. L. McKee, A. Restrepo, L. E. Cano & M. R. Edwards Referencia : Mycopathologia. 1991 Apr;114(1):9-15. DOI (Digital Object Identifier) : 10.1007/BF00436685 PMID : 1857400 En línea : https://link.springer.com/article/10.1007/BF00436685 Enlace permanente : https://hospitalpablotobon.cloudbiteca.com/pmb/opac_css/index.php?lvl=notice_display&id=4361 Scanning electron microscopy of the conidia produced by the mycelial form of Paracoccidioides brasiliensis [documento electrónico] / Maria E. Salazar, ; Ángela Restrepo Moreno, ; Luz Elena Cano Restrepo, . - 1991.
Obra : Mycopathologia
Idioma : Inglés (eng)
Palabras clave : Mycelial conidia Paracoccidioides brasiliensis scanning electron microscopy Resumen : The conidia produced by the mycelial form of Paracoccidioides brasiliensis were examined by scanning electron microscopy for the first time. Several different conidial types were characterized. These included intercalary arthroconidia, several types of septate conidia that are formed from other conidia, pedunculate conidia, and terminal hyphal conidia. In addition, the ultrastructure of the supporting pedestal of the pedunculate conidium was found to be separated from the mother conidium by a septum in some instances, and at other times it was not. Mención de responsabilidad : William A. Samsonoff, M. E. Salazar, M. L. McKee, A. Restrepo, L. E. Cano & M. R. Edwards Referencia : Mycopathologia. 1991 Apr;114(1):9-15. DOI (Digital Object Identifier) : 10.1007/BF00436685 PMID : 1857400 En línea : https://link.springer.com/article/10.1007/BF00436685 Enlace permanente : https://hospitalpablotobon.cloudbiteca.com/pmb/opac_css/index.php?lvl=notice_display&id=4361 Reserva
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Código de barras Número de Ubicación Tipo de medio Ubicación Sección Estado DD000861 AC-1991-003 Archivo digital Producción Científica Artículos científicos Disponible
Título : Inhibition by estrogens of conidium-to-yeast conversion in the fungus Paracoccidioides brasiliensis Tipo de documento : documento electrónico Autores : Maria E. Salazar, ; Ángela Restrepo Moreno, Fecha de publicación : 1988 Títulos uniformes : Infection and Immunity Idioma : Inglés (eng) Resumen : Conidia produced by Paracoccidioides brasiliensis are inhibited by mammalian estrogens in their in vitro conversion into yeast-form cells. This was demonstrated with four different isolates. In these experiments, conversion was reduced to 10.7 and 34.4% of the control values by 17-beta-estradiol at 10(-6) and 10(-8) M, respectively. At the same concentrations, the synthetic estrogen diethylstilbestrol was slightly less inhibitory. In contrast, other sex hormones and analogs, i.e., testosterone, 17-alpha-estradiol, tamoxifen, and hydroxytamoxifen, had no effect on conidium-to-yeast conversion. Previous studies have shown that estrogens similarly inhibit mycelium-to-yeast-form transition in P. brasiliensis. Conidia, and not mycelial fragments, are believed to be the natural infectious propagules. These findings with conidia support the hypothesis that estrogens, affecting the initial host-parasite interactions by suppressing conversion to the parasitic form of the organism, are, at least in part, responsible for the greater resistance of females to paracoccidioidomycosis. Mención de responsabilidad : M E Salazar, A Restrepo, D A Stevens Referencia : Infect Immun. 1988 Mar;56(3):711-3 PMID : 3343055 En línea : https://iai.asm.org/content/56/3/711.long Enlace permanente : https://hospitalpablotobon.cloudbiteca.com/pmb/opac_css/index.php?lvl=notice_display&id=4346 Inhibition by estrogens of conidium-to-yeast conversion in the fungus Paracoccidioides brasiliensis [documento electrónico] / Maria E. Salazar, ; Ángela Restrepo Moreno, . - 1988.
Obra : Infection and Immunity
Idioma : Inglés (eng)
Resumen : Conidia produced by Paracoccidioides brasiliensis are inhibited by mammalian estrogens in their in vitro conversion into yeast-form cells. This was demonstrated with four different isolates. In these experiments, conversion was reduced to 10.7 and 34.4% of the control values by 17-beta-estradiol at 10(-6) and 10(-8) M, respectively. At the same concentrations, the synthetic estrogen diethylstilbestrol was slightly less inhibitory. In contrast, other sex hormones and analogs, i.e., testosterone, 17-alpha-estradiol, tamoxifen, and hydroxytamoxifen, had no effect on conidium-to-yeast conversion. Previous studies have shown that estrogens similarly inhibit mycelium-to-yeast-form transition in P. brasiliensis. Conidia, and not mycelial fragments, are believed to be the natural infectious propagules. These findings with conidia support the hypothesis that estrogens, affecting the initial host-parasite interactions by suppressing conversion to the parasitic form of the organism, are, at least in part, responsible for the greater resistance of females to paracoccidioidomycosis. Mención de responsabilidad : M E Salazar, A Restrepo, D A Stevens Referencia : Infect Immun. 1988 Mar;56(3):711-3 PMID : 3343055 En línea : https://iai.asm.org/content/56/3/711.long Enlace permanente : https://hospitalpablotobon.cloudbiteca.com/pmb/opac_css/index.php?lvl=notice_display&id=4346 Reserva
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Código de barras Número de Ubicación Tipo de medio Ubicación Sección Estado DD000847 AC-1988-002 Archivo digital Producción Científica Artículos científicos Disponible
Título : Morphological study of a variant of Paracoccidioides brasiliensis that exists in the yeast form at room temperature Tipo de documento : documento electrónico Autores : L. A. Villar, ; Maria E. Salazar, ; Ángela Restrepo Moreno, Fecha de publicación : 1988 Títulos uniformes : Journal of Medical and Veterinary Mycology Idioma : Inglés (eng) Resumen : Incubation of Paracoccidioides brasiliensis conidia at 20–25°C (RT) results in two types of colonies, mycelial (M) and yeast (YRT). A study of the latter colonies was undertaken. Conidia were plated in complex (BHI) and chemically-defined media (CDCM), with and without fetal calf serum (FCS). Incubation was carried out at 21°C for 4 weeks. The mean number of YRT colonies was approximately 18·5% on BHI agar. Selected YRT colonies were transferred to liquid CDCM, incubated 7 days at 21°C and transferred to solid media; YRT appearance was preserved in 95% of the colonies if media were FCS-supplemented; otherwise, most colonies reverted to the M form. When FCS was replaced by bovine albumin or by alpha-globulin, 63% and 68% respectively of the colonies obtained after plating YRT cells, became mycelial. Comparative morphologic studies of both YRT and yeasts grown at 37°C suggested that there were no major differences between these two types of yeast cells when size and budding were taken into consideration. The results indicate that in this particular variant, dimorphism is not exclusively temperature-dependent. Mención de responsabilidad : L.A. Villar, M.E. Salazar, A. Restrepo Referencia : J Med Vet Mycol. 1988;26(5):269-76 DOI (Digital Object Identifier) : 10.1080/02681218880000381 PMID : 3236146 En línea : https://academic.oup.com/mmy/article-abstract/26/5/269/895907 Enlace permanente : https://hospitalpablotobon.cloudbiteca.com/pmb/opac_css/index.php?lvl=notice_display&id=4347 Morphological study of a variant of Paracoccidioides brasiliensis that exists in the yeast form at room temperature [documento electrónico] / L. A. Villar, ; Maria E. Salazar, ; Ángela Restrepo Moreno, . - 1988.
Obra : Journal of Medical and Veterinary Mycology
Idioma : Inglés (eng)
Resumen : Incubation of Paracoccidioides brasiliensis conidia at 20–25°C (RT) results in two types of colonies, mycelial (M) and yeast (YRT). A study of the latter colonies was undertaken. Conidia were plated in complex (BHI) and chemically-defined media (CDCM), with and without fetal calf serum (FCS). Incubation was carried out at 21°C for 4 weeks. The mean number of YRT colonies was approximately 18·5% on BHI agar. Selected YRT colonies were transferred to liquid CDCM, incubated 7 days at 21°C and transferred to solid media; YRT appearance was preserved in 95% of the colonies if media were FCS-supplemented; otherwise, most colonies reverted to the M form. When FCS was replaced by bovine albumin or by alpha-globulin, 63% and 68% respectively of the colonies obtained after plating YRT cells, became mycelial. Comparative morphologic studies of both YRT and yeasts grown at 37°C suggested that there were no major differences between these two types of yeast cells when size and budding were taken into consideration. The results indicate that in this particular variant, dimorphism is not exclusively temperature-dependent. Mención de responsabilidad : L.A. Villar, M.E. Salazar, A. Restrepo Referencia : J Med Vet Mycol. 1988;26(5):269-76 DOI (Digital Object Identifier) : 10.1080/02681218880000381 PMID : 3236146 En línea : https://academic.oup.com/mmy/article-abstract/26/5/269/895907 Enlace permanente : https://hospitalpablotobon.cloudbiteca.com/pmb/opac_css/index.php?lvl=notice_display&id=4347 Reserva
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