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Genetic diversity of hepatitis C virus and resistance associated substitutions to direct-acting antiviral treatment in Colombia / Sergio Iván Hoyos Duque ; Juan Carlos Restrepo Gutiérrez  

Público ISBD Título : Genetic diversity of hepatitis C virus and resistance associated substitutions to direct-acting antiviral treatment in Colombia Tipo de documento : documento electrónico Autores : Sergio Iván Hoyos Duque, ; Juan Carlos Restrepo Gutiérrez, Fecha de publicación : 2022 Títulos uniformes : Virus Research Idioma : Inglés (eng) Palabras clave : Hepatitis C virus  Infection  Epidemiology  Genetic diversity  Evolution Resumen : Hepatitis C virus (HCV) infection is one of the leading risk factors for end-stage liver disease development worldwide. This RNA virus displays high genetic diversity with 8 genotypes and 96 subgenotypes with heterogeneous geographical distribution around the world. In this study, we carried out an active case finding of individuals with a history of transfusion events before 1996 in three cities in Colombia. Then, the characterization of the HCV genotypes, subgenotypes, and resistance associate substitutions (RAS) was performed in samples positives for antibodies anti-HCV + from this study population. In addition, samples from PWID and patients with end-stage liver disease submitted to liver transplantation were included in the phylogenetic and RAS analysis. The 5′UTR, NS5A, and NS5B regions of the HCV genome were amplified in serum or liver explants samples. After the edition, assembly, and alignment of the sequences, genotyping through phylogenetic analysis was performed using IQTREE V2.0.5 based on the maximum likelihood approach. The identification of RAS was carried out by alignments based on the reference sequence (GenBank NC_004102). Two hundred sixty individuals with blood transfusion events before 1996 were recruited. The seroprevalence of antibodies anti-HCV was 2.69% in this population. The HCV genotypes 1, 2, and 4 and subgenotypes 1a, 1b, 2a, 4a and 4d were characterized in samples of the study populations. Three RAS (Q30R, C316N, and Y93H) were identified in samples obtained from 2 individuals who received blood transfusion before 1996 and without previous antiviral treatment and 6 samples obtained from patients with end-stage liver disease. Among the 20 samples analyzed, the HCV genotype 1, subgenotype 1b, was the most frequent (60%). We report the first characterization of HCV subgenotypes 4a and 4d and the first RAS identification in patients in Colombia. Mención de responsabilidad : Maria C Lopez-Osorio, José Aldemar Usme-Ciro, José William Martínez, Dioselina Peláez-Carvajal, Javier Hernández, Sergio Hoyos, Juan Carlos Restrepo, Maria-Cristina Navas Referencia : Virus Res. 2022 Sep;318:198847. DOI (Digital Object Identifier) : 10.1016/j.virusres.2022.198847 PMID : 35697300 En línea : https://linkinghub.elsevier.com/retrieve/pii/S0168170222001757 Enlace permanente : https://hospitalpablotobon.cloudbiteca.com/pmb/opac_css/index.php?lvl=notice_display&id=6090 Genetic diversity of hepatitis C virus and resistance associated substitutions to direct-acting antiviral treatment in Colombia [documento electrónico] / Sergio Iván Hoyos Duque, ; Juan Carlos Restrepo Gutiérrez,  . - 2022. Obra : Virus Research Idioma : Inglés (eng) Palabras clave : Hepatitis C virus  Infection  Epidemiology  Genetic diversity  Evolution Resumen : Hepatitis C virus (HCV) infection is one of the leading risk factors for end-stage liver disease development worldwide. This RNA virus displays high genetic diversity with 8 genotypes and 96 subgenotypes with heterogeneous geographical distribution around the world. In this study, we carried out an active case finding of individuals with a history of transfusion events before 1996 in three cities in Colombia. Then, the characterization of the HCV genotypes, subgenotypes, and resistance associate substitutions (RAS) was performed in samples positives for antibodies anti-HCV + from this study population. In addition, samples from PWID and patients with end-stage liver disease submitted to liver transplantation were included in the phylogenetic and RAS analysis. The 5′UTR, NS5A, and NS5B regions of the HCV genome were amplified in serum or liver explants samples. After the edition, assembly, and alignment of the sequences, genotyping through phylogenetic analysis was performed using IQTREE V2.0.5 based on the maximum likelihood approach. The identification of RAS was carried out by alignments based on the reference sequence (GenBank NC_004102). Two hundred sixty individuals with blood transfusion events before 1996 were recruited. The seroprevalence of antibodies anti-HCV was 2.69% in this population. The HCV genotypes 1, 2, and 4 and subgenotypes 1a, 1b, 2a, 4a and 4d were characterized in samples of the study populations. Three RAS (Q30R, C316N, and Y93H) were identified in samples obtained from 2 individuals who received blood transfusion before 1996 and without previous antiviral treatment and 6 samples obtained from patients with end-stage liver disease. Among the 20 samples analyzed, the HCV genotype 1, subgenotype 1b, was the most frequent (60%). We report the first characterization of HCV subgenotypes 4a and 4d and the first RAS identification in patients in Colombia. Mención de responsabilidad : Maria C Lopez-Osorio, José Aldemar Usme-Ciro, José William Martínez, Dioselina Peláez-Carvajal, Javier Hernández, Sergio Hoyos, Juan Carlos Restrepo, Maria-Cristina Navas Referencia : Virus Res. 2022 Sep;318:198847. DOI (Digital Object Identifier) : 10.1016/j.virusres.2022.198847 PMID : 35697300 En línea : https://linkinghub.elsevier.com/retrieve/pii/S0168170222001757 Enlace permanente : https://hospitalpablotobon.cloudbiteca.com/pmb/opac_css/index.php?lvl=notice_display&id=6090

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