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Enlace permanente de la investigaciónInvolvement of extracellular matrix proteins in the course of experimental paracoccidioidomycosis / Beatriz Helena Aristizábal Bernal
Título : Involvement of extracellular matrix proteins in the course of experimental paracoccidioidomycosis Tipo de documento : documento electrónico Autores : Beatriz Helena Aristizábal Bernal, Fecha de publicación : 2008 Títulos uniformes : FEMS Immunology and Medical Microbiology Idioma : Inglés (eng) Palabras clave : Paracoccidioides brasiliensis adhesion laminin fibronectin fibrinogen Resumen : We aimed at determining involvement of extracellular matrix proteins (ECMp) and an ECM-binding adhesin (32-kDa protein) from Paracoccidioides brasiliensis, in the course of experimental paracoccidioidomycosis. BALB/c mice were infected with P. brasiliensis conidia previously incubated with soluble laminin, fibronectin and fibrinogen or a mAb against the fungal adhesin. Inflammatory response, chitin levels and cytokine production at different postinfection periods were determined. Chitin was significantly decreased in lungs of mice infected with ECMp-treated conidia when compared with controls at week 8, especially with laminin and fibrinogen. Contrariwise, when animals were infected with mAb-treated conidia no differences in chitin content were found. The observed inflammatory reaction in lungs was equivalent in all cases. IFN-g increased significantly in lungs from mice infected with soluble ECMp – (at day 4 and week 12) or mAb-treated conidia (at week 12) when compared with animals infected with untreated conidia. Significant increased levels of tumour necrosis factor-a were observed at 8 weeks in animals infected with ECMp-treated conidia while no differences were observed during the remaining periods. These findings point toward an inhibitory effect of ECMp on P. brasiliensis conidia infectivity and suggest that these proteins may interfere with conidia initial adhesion to host tissues probably modulating the immune response in paracoccidioidomycosis. Mención de responsabilidad : Angel González, Beatriz L. Gómez, Cesar Muñozm Beatriz H. Aristizabal, Angela Restrepo, Andrew J. Hamilton, Luz E. Cano Referencia : FEMS Immunol Med Microbiol. 2008 Jun;53(1):114-25. DOI (Digital Object Identifier) : 10.1111/j.1574-695X.2008.00411.x PMID : 18400008 En línea : https://onlinelibrary.wiley.com/doi/10.1111/j.1574-695X.2008.00411.x Enlace permanente : https://hospitalpablotobon.cloudbiteca.com/pmb/opac_css/index.php?lvl=notice_display&id=3465 Involvement of extracellular matrix proteins in the course of experimental paracoccidioidomycosis [documento electrónico] / Beatriz Helena Aristizábal Bernal, . - 2008.
Obra : FEMS Immunology and Medical Microbiology
Idioma : Inglés (eng)
Palabras clave : Paracoccidioides brasiliensis adhesion laminin fibronectin fibrinogen Resumen : We aimed at determining involvement of extracellular matrix proteins (ECMp) and an ECM-binding adhesin (32-kDa protein) from Paracoccidioides brasiliensis, in the course of experimental paracoccidioidomycosis. BALB/c mice were infected with P. brasiliensis conidia previously incubated with soluble laminin, fibronectin and fibrinogen or a mAb against the fungal adhesin. Inflammatory response, chitin levels and cytokine production at different postinfection periods were determined. Chitin was significantly decreased in lungs of mice infected with ECMp-treated conidia when compared with controls at week 8, especially with laminin and fibrinogen. Contrariwise, when animals were infected with mAb-treated conidia no differences in chitin content were found. The observed inflammatory reaction in lungs was equivalent in all cases. IFN-g increased significantly in lungs from mice infected with soluble ECMp – (at day 4 and week 12) or mAb-treated conidia (at week 12) when compared with animals infected with untreated conidia. Significant increased levels of tumour necrosis factor-a were observed at 8 weeks in animals infected with ECMp-treated conidia while no differences were observed during the remaining periods. These findings point toward an inhibitory effect of ECMp on P. brasiliensis conidia infectivity and suggest that these proteins may interfere with conidia initial adhesion to host tissues probably modulating the immune response in paracoccidioidomycosis. Mención de responsabilidad : Angel González, Beatriz L. Gómez, Cesar Muñozm Beatriz H. Aristizabal, Angela Restrepo, Andrew J. Hamilton, Luz E. Cano Referencia : FEMS Immunol Med Microbiol. 2008 Jun;53(1):114-25. DOI (Digital Object Identifier) : 10.1111/j.1574-695X.2008.00411.x PMID : 18400008 En línea : https://onlinelibrary.wiley.com/doi/10.1111/j.1574-695X.2008.00411.x Enlace permanente : https://hospitalpablotobon.cloudbiteca.com/pmb/opac_css/index.php?lvl=notice_display&id=3465 Reserva
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Código de barras Número de Ubicación Tipo de medio Ubicación Sección Estado DD000033 AC-2008-006 Archivo digital Producción Científica Artículos científicos Disponible 
Título : Lysozyme plays a dual role against the dimorphic fungus Paracoccidioides brasiliensis Tipo de documento : documento electrónico Autores : Beatriz Helena Aristizábal Bernal, Fecha de publicación : 2008 Títulos uniformes : Revista do Instituto de Medicina Tropical de São Paulo Idioma : Inglés (eng) Palabras clave : Lysozyme TNF- Alveolar macrophages Paracoccidioides brasiliensis Resumen : In order to determine the role of lysozyme, an antimicrobial peptide belonging to the innate immune system, against the dimorphic fungus Paracoccidioides brasiliensis, co-cultures of the MH-S murine alveolar macrophages cell line with P. brasiliensis conidia were done; assays to evaluate the effect of physiological and inflammatory concentrations of lysozyme directly on the fungus life cycle were also undertaken. We observed that TNF-α-activated macrophages significantly inhibited the conidia to yeast transition (p = 0.0043) and exerted an important fungicidal effect (p = 0.0044), killing 27% more fungal propagules in comparison with controls. Nonetheless, after adding a selective inhibitor of lysozyme, the fungicidal effect was reverted. When P. brasiliensis propagules were exposed directly to different concentrations of lysozyme, a dual effect was observed. Physiologic concentrations of the enzyme facilitated the conidia-to-yeast transition process (p Mención de responsabilidad : Damaris Lopera; Beatriz H. Aristizabal; Angela Restrepo; Luz Elena Cano; Ángel González Referencia : Rev Inst Med Trop Sao Paulo. May-Jun 2008;50(3):169-75. DOI (Digital Object Identifier) : 10.1590/S0036-46652008000300008 PMID : 18604417 Derechos de uso : CC BY-NC En línea : http://www.scielo.br/scielo.php?script=sci_abstract&pid=S0036-46652008000300008 Enlace permanente : https://hospitalpablotobon.cloudbiteca.com/pmb/opac_css/index.php?lvl=notice_display&id=4438 Lysozyme plays a dual role against the dimorphic fungus Paracoccidioides brasiliensis [documento electrónico] / Beatriz Helena Aristizábal Bernal, . - 2008.
Obra : Revista do Instituto de Medicina Tropical de São Paulo
Idioma : Inglés (eng)
Palabras clave : Lysozyme TNF- Alveolar macrophages Paracoccidioides brasiliensis Resumen : In order to determine the role of lysozyme, an antimicrobial peptide belonging to the innate immune system, against the dimorphic fungus Paracoccidioides brasiliensis, co-cultures of the MH-S murine alveolar macrophages cell line with P. brasiliensis conidia were done; assays to evaluate the effect of physiological and inflammatory concentrations of lysozyme directly on the fungus life cycle were also undertaken. We observed that TNF-α-activated macrophages significantly inhibited the conidia to yeast transition (p = 0.0043) and exerted an important fungicidal effect (p = 0.0044), killing 27% more fungal propagules in comparison with controls. Nonetheless, after adding a selective inhibitor of lysozyme, the fungicidal effect was reverted. When P. brasiliensis propagules were exposed directly to different concentrations of lysozyme, a dual effect was observed. Physiologic concentrations of the enzyme facilitated the conidia-to-yeast transition process (p Mención de responsabilidad : Damaris Lopera; Beatriz H. Aristizabal; Angela Restrepo; Luz Elena Cano; Ángel González Referencia : Rev Inst Med Trop Sao Paulo. May-Jun 2008;50(3):169-75. DOI (Digital Object Identifier) : 10.1590/S0036-46652008000300008 PMID : 18604417 Derechos de uso : CC BY-NC En línea : http://www.scielo.br/scielo.php?script=sci_abstract&pid=S0036-46652008000300008 Enlace permanente : https://hospitalpablotobon.cloudbiteca.com/pmb/opac_css/index.php?lvl=notice_display&id=4438 Reserva
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Código de barras Número de Ubicación Tipo de medio Ubicación Sección Estado DD000938 AC-2008-025 Archivo digital Producción Científica Artículos científicos Disponible Documentos electrónicos
2008-025.pdfAdobe Acrobat PDF
Título : Pulmonary paracoccidioidomycosis Tipo de documento : documento electrónico Autores : Carlos Andrés Agudelo, Fecha de publicación : 2008 Títulos uniformes : Seminars in Respiratory and Critical Care Medicine Idioma : Inglés (eng) Palabras clave : Paracoccidioidomycosis Paracoccidioides brasiliensis clinical presentations diagnosis immunopathology treatment Resumen : Paracoccidioidomycosis (formerly known as South American blastomycosis) is produced by the thermally dimorphic fungus Paracoccidioides brasiliensis. Most often this mycosis runs a chronic progressive course affecting preferentially the lungs followed by the skin, mucous membranes, adrenals, and reticuloendothelial organs. Acute-subacute presentations can be observed in children and immunosuppressed patients. Occasionally, self-limited infections have been documented. Two types of clinical presentations are described, the acute-subacute (juvenile) and the chronic (adult) forms of the disease. Paracoccidioidomycosis predominates in adult males (13:1); this gender difference is not observed in children or adolescents. The mycosis is limited geographically to various Latin American countries, with the greatest number of cases originating in Brazil, The fungus's natural habitat has not been precisely defined, although it is supposed to be a soil-inhabiting microorganism. No outbreaks have been reported. P. brasiliensis is capable of entering into prolonged periods of latency as is demonstrated by its diagnosis in patients who have moved outside the recognized endemic areas. This review updates clinicians and laboratory workers on the characteristics of a mycosis seldom reported outside of the Latin American countries. Mención de responsabilidad : Angela Restrepo, Gil Benard, Cláudio C. de Castro, Carlos A. Agudelo, Angela M. Tobón Referencia : Semin Respir Crit Care Med. 2008 Apr;29(2):182-97. DOI (Digital Object Identifier) : 10.1055/s-2008-1063857 PMID : 18366000 En línea : https://www.thieme-connect.de/products/ejournals/abstract/10.1055/s-2008-1063857 Enlace permanente : https://hospitalpablotobon.cloudbiteca.com/pmb/opac_css/index.php?lvl=notice_display&id=4441 Pulmonary paracoccidioidomycosis [documento electrónico] / Carlos Andrés Agudelo, . - 2008.
Obra : Seminars in Respiratory and Critical Care Medicine
Idioma : Inglés (eng)
Palabras clave : Paracoccidioidomycosis Paracoccidioides brasiliensis clinical presentations diagnosis immunopathology treatment Resumen : Paracoccidioidomycosis (formerly known as South American blastomycosis) is produced by the thermally dimorphic fungus Paracoccidioides brasiliensis. Most often this mycosis runs a chronic progressive course affecting preferentially the lungs followed by the skin, mucous membranes, adrenals, and reticuloendothelial organs. Acute-subacute presentations can be observed in children and immunosuppressed patients. Occasionally, self-limited infections have been documented. Two types of clinical presentations are described, the acute-subacute (juvenile) and the chronic (adult) forms of the disease. Paracoccidioidomycosis predominates in adult males (13:1); this gender difference is not observed in children or adolescents. The mycosis is limited geographically to various Latin American countries, with the greatest number of cases originating in Brazil, The fungus's natural habitat has not been precisely defined, although it is supposed to be a soil-inhabiting microorganism. No outbreaks have been reported. P. brasiliensis is capable of entering into prolonged periods of latency as is demonstrated by its diagnosis in patients who have moved outside the recognized endemic areas. This review updates clinicians and laboratory workers on the characteristics of a mycosis seldom reported outside of the Latin American countries. Mención de responsabilidad : Angela Restrepo, Gil Benard, Cláudio C. de Castro, Carlos A. Agudelo, Angela M. Tobón Referencia : Semin Respir Crit Care Med. 2008 Apr;29(2):182-97. DOI (Digital Object Identifier) : 10.1055/s-2008-1063857 PMID : 18366000 En línea : https://www.thieme-connect.de/products/ejournals/abstract/10.1055/s-2008-1063857 Enlace permanente : https://hospitalpablotobon.cloudbiteca.com/pmb/opac_css/index.php?lvl=notice_display&id=4441 Reserva
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Código de barras Número de Ubicación Tipo de medio Ubicación Sección Estado DD000941 AC-2008-028 Archivo digital Producción Científica Artículos científicos Disponible
Título : Scanning electron microscopy of the conidia produced by the mycelial form of Paracoccidioides brasiliensis Tipo de documento : documento electrónico Autores : Maria E. Salazar, ; Ángela Restrepo Moreno, ; Luz Elena Cano Restrepo, Fecha de publicación : 1991 Títulos uniformes : Mycopathologia Idioma : Inglés (eng) Palabras clave : Mycelial conidia Paracoccidioides brasiliensis scanning electron microscopy Resumen : The conidia produced by the mycelial form of Paracoccidioides brasiliensis were examined by scanning electron microscopy for the first time. Several different conidial types were characterized. These included intercalary arthroconidia, several types of septate conidia that are formed from other conidia, pedunculate conidia, and terminal hyphal conidia. In addition, the ultrastructure of the supporting pedestal of the pedunculate conidium was found to be separated from the mother conidium by a septum in some instances, and at other times it was not. Mención de responsabilidad : William A. Samsonoff, M. E. Salazar, M. L. McKee, A. Restrepo, L. E. Cano & M. R. Edwards Referencia : Mycopathologia. 1991 Apr;114(1):9-15. DOI (Digital Object Identifier) : 10.1007/BF00436685 PMID : 1857400 En línea : https://link.springer.com/article/10.1007/BF00436685 Enlace permanente : https://hospitalpablotobon.cloudbiteca.com/pmb/opac_css/index.php?lvl=notice_display&id=4361 Scanning electron microscopy of the conidia produced by the mycelial form of Paracoccidioides brasiliensis [documento electrónico] / Maria E. Salazar, ; Ángela Restrepo Moreno, ; Luz Elena Cano Restrepo, . - 1991.
Obra : Mycopathologia
Idioma : Inglés (eng)
Palabras clave : Mycelial conidia Paracoccidioides brasiliensis scanning electron microscopy Resumen : The conidia produced by the mycelial form of Paracoccidioides brasiliensis were examined by scanning electron microscopy for the first time. Several different conidial types were characterized. These included intercalary arthroconidia, several types of septate conidia that are formed from other conidia, pedunculate conidia, and terminal hyphal conidia. In addition, the ultrastructure of the supporting pedestal of the pedunculate conidium was found to be separated from the mother conidium by a septum in some instances, and at other times it was not. Mención de responsabilidad : William A. Samsonoff, M. E. Salazar, M. L. McKee, A. Restrepo, L. E. Cano & M. R. Edwards Referencia : Mycopathologia. 1991 Apr;114(1):9-15. DOI (Digital Object Identifier) : 10.1007/BF00436685 PMID : 1857400 En línea : https://link.springer.com/article/10.1007/BF00436685 Enlace permanente : https://hospitalpablotobon.cloudbiteca.com/pmb/opac_css/index.php?lvl=notice_display&id=4361 Reserva
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Código de barras Número de Ubicación Tipo de medio Ubicación Sección Estado DD000861 AC-1991-003 Archivo digital Producción Científica Artículos científicos Disponible
Título : Virulence of Paracoccidioides brasiliensis: The influence of in vitro passage and storage Tipo de documento : documento electrónico Autores : Ángela Restrepo Moreno, Fecha de publicación : 1990 Títulos uniformes : Mycopathologia Idioma : Inglés (eng) Palabras clave : Paracoccidioides brasiliensis Paracoccidioidomycosis passage in vitro virulence Resumen : Stability of virulence in P. brasiliensis isolates was studied with respect to the in vitro culture history and methods used for storage. Virulence in yeast-form P. brasiliensis isolates was tested in a chronic pulmonary murine model of paracoccidioidomycosis where progression of disease was quantitated in terms of colony forming units recoverable from lungs. Four isolates of P. brasiliensis, including recently isolated from patients or experimental animals, caused chronic progressive disease. Two isolates with a history of subculturing showed attenuation by causing resolving but chronic disease. An attenuated isolate became avirulent subsequent to 15 more years of subculturing. These findings suggest that virulence of P. brasiliensis can be attenuated or lost subsequent to cycles of subculturing over long periods. Our data suggest that the use of fresh P. brasiliensis isolates may be needed to provide reproducible virulence for experimental systems. Mención de responsabilidad : Elmer Brummer, Angela Restrepo, Linda H. Hanson & David A. Stevens Referencia : Mycopathologia. 1990 Jan;109(1):13-7. DOI (Digital Object Identifier) : 10.1007/BF00437001 PMID : 2139178 En línea : https://link.springer.com/article/10.1007%2FBF00437001 Enlace permanente : https://hospitalpablotobon.cloudbiteca.com/pmb/opac_css/index.php?lvl=notice_display&id=4358 Virulence of Paracoccidioides brasiliensis: The influence of in vitro passage and storage [documento electrónico] / Ángela Restrepo Moreno, . - 1990.
Obra : Mycopathologia
Idioma : Inglés (eng)
Palabras clave : Paracoccidioides brasiliensis Paracoccidioidomycosis passage in vitro virulence Resumen : Stability of virulence in P. brasiliensis isolates was studied with respect to the in vitro culture history and methods used for storage. Virulence in yeast-form P. brasiliensis isolates was tested in a chronic pulmonary murine model of paracoccidioidomycosis where progression of disease was quantitated in terms of colony forming units recoverable from lungs. Four isolates of P. brasiliensis, including recently isolated from patients or experimental animals, caused chronic progressive disease. Two isolates with a history of subculturing showed attenuation by causing resolving but chronic disease. An attenuated isolate became avirulent subsequent to 15 more years of subculturing. These findings suggest that virulence of P. brasiliensis can be attenuated or lost subsequent to cycles of subculturing over long periods. Our data suggest that the use of fresh P. brasiliensis isolates may be needed to provide reproducible virulence for experimental systems. Mención de responsabilidad : Elmer Brummer, Angela Restrepo, Linda H. Hanson & David A. Stevens Referencia : Mycopathologia. 1990 Jan;109(1):13-7. DOI (Digital Object Identifier) : 10.1007/BF00437001 PMID : 2139178 En línea : https://link.springer.com/article/10.1007%2FBF00437001 Enlace permanente : https://hospitalpablotobon.cloudbiteca.com/pmb/opac_css/index.php?lvl=notice_display&id=4358 Reserva
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Código de barras Número de Ubicación Tipo de medio Ubicación Sección Estado DD000858 AC-1990-003 Archivo digital Producción Científica Artículos científicos Disponible
