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PLoS One
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Documentos disponibles con este título uniforme (4)
Clasificado(s) por (Año de edición descendente) Refinar búsquedaMolecular characterization of occult hepatitis B virus infection in patients with end-stage liver disease in Colombia / Juan Carlos Restrepo Gutiérrez ; Sergio Iván Hoyos Duque
Título : Molecular characterization of occult hepatitis B virus infection in patients with end-stage liver disease in Colombia Tipo de documento : documento electrónico Autores : Juan Carlos Restrepo Gutiérrez, ; Sergio Iván Hoyos Duque, Fecha de publicación : 2017 Títulos uniformes : PLoS One Idioma : Inglés (eng) Resumen : Background: Hepatitis B virus (HBV) occult infection (OBI) is a risk factor to be taken into account in transfusion, hemodialysis and organ transplantation. The aim of this study was to identify and characterize at the molecular level OBI cases in patients with end-stage liver disease. Methods: Sixty-six liver samples were obtained from patients with diagnosis of end-stage liver disease submitted to liver transplantation in Medellin (North West, Colombia). Samples obtained from patients who were negative for the surface antigen of HBV (n = 50) were tested for viral DNA detection by nested PCR for ORFs S, C, and X and confirmed by Southern-Blot. OBI cases were analyzed by sequencing the viral genome to determine the genotype and mutations; additionally, viral genome integration events were examined by the Alu-PCR technique. Results:In five cases out of 50 patients (10%) the criteria for OBI was confirmed. HBV genotype F (subgenotypes F1 and F3), genotype A and genotype D were characterized in liver samples. Three integration events in chromosomes 5q14.1, 16p13 and 20q12 affecting Receptor-type tyrosine-protein phosphatase T, Ras Protein Specific Guanine Nucleotide Releasing Factor 2, and the zinc finger 263 genes were identified in two OBI cases. Sequence analysis of the viral genome of the 5 OBI cases showed several punctual missense and nonsense mutations affecting ORFs S, P, Core and X. Conclusions: This is the first characterization of OBI in patients with end-stage liver disease in Colombia. The OBI cases were identified in patients with HCV infection or cryptogenic cirrhosis. The integration events (5q14.1, 16p13 and 20q12) described in this study have not been previously reported. Further studies are required to validate the role of mutations and integration events in OBI pathogenesis Mención de responsabilidad : Julio Cesar Rendon, Fabian Cortes-Mancera, Juan Carlos Restrepo-Gutierrez, Sergio Hoyos, Maria-Cristina Navas Referencia : PLoS One. 2017 Jul 7;12(7):e0180447. DOI (Digital Object Identifier) : 10.1371/journal.pone.0180447 PMID : 28686707 Derechos de uso : CC BY En línea : https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0180447 Enlace permanente : https://hospitalpablotobon.cloudbiteca.com/pmb/opac_css/index.php?lvl=notice_display&id=4051 Molecular characterization of occult hepatitis B virus infection in patients with end-stage liver disease in Colombia [documento electrónico] / Juan Carlos Restrepo Gutiérrez, ; Sergio Iván Hoyos Duque, . - 2017.
Obra : PLoS One
Idioma : Inglés (eng)
Resumen : Background: Hepatitis B virus (HBV) occult infection (OBI) is a risk factor to be taken into account in transfusion, hemodialysis and organ transplantation. The aim of this study was to identify and characterize at the molecular level OBI cases in patients with end-stage liver disease. Methods: Sixty-six liver samples were obtained from patients with diagnosis of end-stage liver disease submitted to liver transplantation in Medellin (North West, Colombia). Samples obtained from patients who were negative for the surface antigen of HBV (n = 50) were tested for viral DNA detection by nested PCR for ORFs S, C, and X and confirmed by Southern-Blot. OBI cases were analyzed by sequencing the viral genome to determine the genotype and mutations; additionally, viral genome integration events were examined by the Alu-PCR technique. Results:In five cases out of 50 patients (10%) the criteria for OBI was confirmed. HBV genotype F (subgenotypes F1 and F3), genotype A and genotype D were characterized in liver samples. Three integration events in chromosomes 5q14.1, 16p13 and 20q12 affecting Receptor-type tyrosine-protein phosphatase T, Ras Protein Specific Guanine Nucleotide Releasing Factor 2, and the zinc finger 263 genes were identified in two OBI cases. Sequence analysis of the viral genome of the 5 OBI cases showed several punctual missense and nonsense mutations affecting ORFs S, P, Core and X. Conclusions: This is the first characterization of OBI in patients with end-stage liver disease in Colombia. The OBI cases were identified in patients with HCV infection or cryptogenic cirrhosis. The integration events (5q14.1, 16p13 and 20q12) described in this study have not been previously reported. Further studies are required to validate the role of mutations and integration events in OBI pathogenesis Mención de responsabilidad : Julio Cesar Rendon, Fabian Cortes-Mancera, Juan Carlos Restrepo-Gutierrez, Sergio Hoyos, Maria-Cristina Navas Referencia : PLoS One. 2017 Jul 7;12(7):e0180447. DOI (Digital Object Identifier) : 10.1371/journal.pone.0180447 PMID : 28686707 Derechos de uso : CC BY En línea : https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0180447 Enlace permanente : https://hospitalpablotobon.cloudbiteca.com/pmb/opac_css/index.php?lvl=notice_display&id=4051 Reserva
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Código de barras Número de Ubicación Tipo de medio Ubicación Sección Estado DD000651 AC-2017-040 Archivo digital Producción Científica Artículos científicos Disponible Documentos electrónicos
2017-040.pdfAdobe Acrobat PDF
Título : Hepatitis E Virus Genotype 3 in Colombia: Survey in Patients with Clinical Diagnosis of Viral Hepatitis Tipo de documento : documento electrónico Autores : María Elsy Sepúlveda Hincapie, ; Juan Carlos Restrepo Gutiérrez, ; Sergio Jaramillo Velásquez, Fecha de publicación : 2016 Títulos uniformes : PLoS One Idioma : Inglés (eng) Resumen : Background: Hepatitis E virus is a major cause of outbreaks as well as sporadic hepatitis cases worldwide. The epidemiology of this enterically transmitted infection differs between developing and developed countries. The aims of this study were to describe HEV infection in Colombian patients and to characterize the genotype. Methods: A prospective study was carried out on 40 patients aged over 15 with a clinical diagnosis of viral hepatitis, recruited from five primary health units in the city of Medellin, Colombia. Fecal samples obtained from the 40 consecutives cases were analyzed for HEV RNA using nested reverse transcription PCR for both ORF1 and ORF2-3. The amplicons were sequenced for phylogenetic analyses. Results: Nine (22.5%) cases of HEV infection were identified in the study population. Three HEV strains obtained from patients were classified as genotype 3. No significant association was found between cases of Hepatitis E and the variables water drinking source, garbage collection system and contact with pigs. Conclusions: This is the first prospective study of hepatitis E in Colombian patients. The circulation of the genotype 3 in this population is predictable considering the reports of the region and the identification of this genotype from pigs in the state of Antioquia, of which Medellin is the capital. Further studies are necessary to establish whether zoonotic transmission of HEV is important in Colombia. Mención de responsabilidad : Julio Rendon, Maria Cristina Hoyos, Diana di Filippo, Fabian Cortes-Mancera, Carolina Mantilla, Maria Mercedes Velasquez, Maria Elsy Sepulveda, Juan Carlos Restrepo, Sergio Jaramillo, Maria Patricia Arbelaez, Gonzalo Correa, Maria-Cristina Navas Referencia : PLoS One. 2016 Feb 17;11(2):e0148417. DOI (Digital Object Identifier) : 10.1371/journal.pone.0148417 PMID : 26886728 Derechos de uso : CC BY En línea : https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0148417 Enlace permanente : https://hospitalpablotobon.cloudbiteca.com/pmb/opac_css/index.php?lvl=notice_display&id=3960 Hepatitis E Virus Genotype 3 in Colombia: Survey in Patients with Clinical Diagnosis of Viral Hepatitis [documento electrónico] / María Elsy Sepúlveda Hincapie, ; Juan Carlos Restrepo Gutiérrez, ; Sergio Jaramillo Velásquez, . - 2016.
Obra : PLoS One
Idioma : Inglés (eng)
Resumen : Background: Hepatitis E virus is a major cause of outbreaks as well as sporadic hepatitis cases worldwide. The epidemiology of this enterically transmitted infection differs between developing and developed countries. The aims of this study were to describe HEV infection in Colombian patients and to characterize the genotype. Methods: A prospective study was carried out on 40 patients aged over 15 with a clinical diagnosis of viral hepatitis, recruited from five primary health units in the city of Medellin, Colombia. Fecal samples obtained from the 40 consecutives cases were analyzed for HEV RNA using nested reverse transcription PCR for both ORF1 and ORF2-3. The amplicons were sequenced for phylogenetic analyses. Results: Nine (22.5%) cases of HEV infection were identified in the study population. Three HEV strains obtained from patients were classified as genotype 3. No significant association was found between cases of Hepatitis E and the variables water drinking source, garbage collection system and contact with pigs. Conclusions: This is the first prospective study of hepatitis E in Colombian patients. The circulation of the genotype 3 in this population is predictable considering the reports of the region and the identification of this genotype from pigs in the state of Antioquia, of which Medellin is the capital. Further studies are necessary to establish whether zoonotic transmission of HEV is important in Colombia. Mención de responsabilidad : Julio Rendon, Maria Cristina Hoyos, Diana di Filippo, Fabian Cortes-Mancera, Carolina Mantilla, Maria Mercedes Velasquez, Maria Elsy Sepulveda, Juan Carlos Restrepo, Sergio Jaramillo, Maria Patricia Arbelaez, Gonzalo Correa, Maria-Cristina Navas Referencia : PLoS One. 2016 Feb 17;11(2):e0148417. DOI (Digital Object Identifier) : 10.1371/journal.pone.0148417 PMID : 26886728 Derechos de uso : CC BY En línea : https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0148417 Enlace permanente : https://hospitalpablotobon.cloudbiteca.com/pmb/opac_css/index.php?lvl=notice_display&id=3960 Reserva
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Código de barras Número de Ubicación Tipo de medio Ubicación Sección Estado DD000546 AC-2016-021 Archivo digital Producción Científica Artículos científicos Disponible Documentos electrónicos
2016-021.pdfAdobe Acrobat PDF
Título : Risk factors for vitamin D deficiency among veterans with and without HIV infection Tipo de documento : documento electrónico Autores : Alicia Inés Hidrón Botero, Fecha de publicación : 2015 Títulos uniformes : PLoS One Idioma : Inglés (eng) Resumen : Objectives: We aimed to describe and compare the prevalence of vitamin D deficiency between HIV-negative and HIV-infected veterans in the southern United States, and to determine risk factors for vitamin D deficiency for HIV infected patients. Methods: Cross-sectional, retrospective study including all patients followed at the Atlanta VA Medical Center with the first 25 hydroxyvitamin D [25(OH)D] level determined between January 2007 and August 2010. Multivariate logistic regression analysis was used to determine risk factors associated with vitamin D deficiency ( Mención de responsabilidad : Alicia I Hidron, Brittany Hill, Jodie L Guest, David Rimland Referencia : PLoS One. 2015 Apr 21;10(4):e0124168. DOI (Digital Object Identifier) : 10.1371/journal.pone.0124168 PMID : 25898185 Derechos de uso : CC BY En línea : https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0124168 Enlace permanente : https://hospitalpablotobon.cloudbiteca.com/pmb/opac_css/index.php?lvl=notice_display&id=3904 Risk factors for vitamin D deficiency among veterans with and without HIV infection [documento electrónico] / Alicia Inés Hidrón Botero, . - 2015.
Obra : PLoS One
Idioma : Inglés (eng)
Resumen : Objectives: We aimed to describe and compare the prevalence of vitamin D deficiency between HIV-negative and HIV-infected veterans in the southern United States, and to determine risk factors for vitamin D deficiency for HIV infected patients. Methods: Cross-sectional, retrospective study including all patients followed at the Atlanta VA Medical Center with the first 25 hydroxyvitamin D [25(OH)D] level determined between January 2007 and August 2010. Multivariate logistic regression analysis was used to determine risk factors associated with vitamin D deficiency ( Mención de responsabilidad : Alicia I Hidron, Brittany Hill, Jodie L Guest, David Rimland Referencia : PLoS One. 2015 Apr 21;10(4):e0124168. DOI (Digital Object Identifier) : 10.1371/journal.pone.0124168 PMID : 25898185 Derechos de uso : CC BY En línea : https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0124168 Enlace permanente : https://hospitalpablotobon.cloudbiteca.com/pmb/opac_css/index.php?lvl=notice_display&id=3904 Reserva
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Código de barras Número de Ubicación Tipo de medio Ubicación Sección Estado DD000485 AC-2015-038 Archivo digital Producción Científica Artículos científicos Disponible Documentos electrónicos
2015-038.PDFAdobe Acrobat PDF
Título : CC8 MRSA strains harboring SCCmec type IVc are predominant in Colombian hospitals Tipo de documento : documento electrónico Autores : Andrea Victoria Restrepo Gouzy, ; Carlos Guillermo Garcés Samudio, Fecha de publicación : 2012 Títulos uniformes : PLoS One Idioma : Inglés (eng) Resumen : Background: Recent reports highlight the incursion of community-associated MRSA within healthcare settings. However, knowledge of this phenomenon remains limited in Latin America. The aim of this study was to evaluate the molecular epidemiology of MRSA in three tertiary-care hospitals in Medellín, Colombia. Methods: An observational cross-sectional study was conducted from 2008–2010. MRSA infections were classified as either community-associated (CA-MRSA) or healthcare-associated (HA-MRSA), with HA-MRSA further classified as hospital-onset (HAHO-MRSA) or community-onset (HACO-MRSA) according to standard epidemiological definitions established by the U.S. Centers for Disease Control and Prevention (CDC). Genotypic analysis included SCCmec typing, spa typing, PFGE and MLST. Results: Out of 538 total MRSA isolates, 68 (12.6%) were defined as CA-MRSA, 243 (45.2%) as HACO-MRSA and 227 (42.2%) as HAHO-MRSA. The majority harbored SCCmec type IVc (306, 58.7%), followed by SCCmec type I (174, 33.4%). The prevalence of type IVc among CA-, HACO- and HAHO-MRSA isolates was 92.4%, 65.1% and 43.6%, respectively. From 2008 to 2010, the prevalence of type IVc-bearing strains increased significantly, from 50.0% to 68.2% (p = 0.004). Strains harboring SCCmec IVc were mainly associated with spa types t1610, t008 and t024 (MLST clonal complex 8), while PFGE confirmed that the t008 and t1610 strains were closely related to the USA300-0114 CA-MRSA clone. Notably, strains belonging to these three spa types exhibited high levels of tetracycline resistance (45.9%). Conclusion: CC8 MRSA strains harboring SCCmec type IVc are becoming predominant in Medellín hospitals, displacing previously reported CC5 HA-MRSA clones. Based on shared characteristics including SCCmec IVc, absence of the ACME element and tetracycline resistance, the USA300-related isolates in this study are most likely related to USA300-LV, the recently-described ‘Latin American variant’ of USA300. Mención de responsabilidad : J Natalia Jiménez, Ana M Ocampo, Johanna M Vanegas, Erika A Rodriguez, José R Mediavilla, Liang Chen, Carlos E Muskus, Lázaro A Vélez, Carlos Rojas, Andrea V Restrepo, Sigifredo Ospina, Carlos Garcés, Liliana Franco, Pablo Bifani, Barry N Kreiswirth, Margarita M Correa Referencia : PLoS One. 2012;7(6):e38576. DOI (Digital Object Identifier) : 10.1371/journal.pone.0038576 PMID : 22745670 Derechos de uso : CC BY En línea : https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0038576 Enlace permanente : https://hospitalpablotobon.cloudbiteca.com/pmb/opac_css/index.php?lvl=notice_display&id=4504 CC8 MRSA strains harboring SCCmec type IVc are predominant in Colombian hospitals [documento electrónico] / Andrea Victoria Restrepo Gouzy, ; Carlos Guillermo Garcés Samudio, . - 2012.
Obra : PLoS One
Idioma : Inglés (eng)
Resumen : Background: Recent reports highlight the incursion of community-associated MRSA within healthcare settings. However, knowledge of this phenomenon remains limited in Latin America. The aim of this study was to evaluate the molecular epidemiology of MRSA in three tertiary-care hospitals in Medellín, Colombia. Methods: An observational cross-sectional study was conducted from 2008–2010. MRSA infections were classified as either community-associated (CA-MRSA) or healthcare-associated (HA-MRSA), with HA-MRSA further classified as hospital-onset (HAHO-MRSA) or community-onset (HACO-MRSA) according to standard epidemiological definitions established by the U.S. Centers for Disease Control and Prevention (CDC). Genotypic analysis included SCCmec typing, spa typing, PFGE and MLST. Results: Out of 538 total MRSA isolates, 68 (12.6%) were defined as CA-MRSA, 243 (45.2%) as HACO-MRSA and 227 (42.2%) as HAHO-MRSA. The majority harbored SCCmec type IVc (306, 58.7%), followed by SCCmec type I (174, 33.4%). The prevalence of type IVc among CA-, HACO- and HAHO-MRSA isolates was 92.4%, 65.1% and 43.6%, respectively. From 2008 to 2010, the prevalence of type IVc-bearing strains increased significantly, from 50.0% to 68.2% (p = 0.004). Strains harboring SCCmec IVc were mainly associated with spa types t1610, t008 and t024 (MLST clonal complex 8), while PFGE confirmed that the t008 and t1610 strains were closely related to the USA300-0114 CA-MRSA clone. Notably, strains belonging to these three spa types exhibited high levels of tetracycline resistance (45.9%). Conclusion: CC8 MRSA strains harboring SCCmec type IVc are becoming predominant in Medellín hospitals, displacing previously reported CC5 HA-MRSA clones. Based on shared characteristics including SCCmec IVc, absence of the ACME element and tetracycline resistance, the USA300-related isolates in this study are most likely related to USA300-LV, the recently-described ‘Latin American variant’ of USA300. Mención de responsabilidad : J Natalia Jiménez, Ana M Ocampo, Johanna M Vanegas, Erika A Rodriguez, José R Mediavilla, Liang Chen, Carlos E Muskus, Lázaro A Vélez, Carlos Rojas, Andrea V Restrepo, Sigifredo Ospina, Carlos Garcés, Liliana Franco, Pablo Bifani, Barry N Kreiswirth, Margarita M Correa Referencia : PLoS One. 2012;7(6):e38576. DOI (Digital Object Identifier) : 10.1371/journal.pone.0038576 PMID : 22745670 Derechos de uso : CC BY En línea : https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0038576 Enlace permanente : https://hospitalpablotobon.cloudbiteca.com/pmb/opac_css/index.php?lvl=notice_display&id=4504 Reserva
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Código de barras Número de Ubicación Tipo de medio Ubicación Sección Estado DD001003 AC-2012-092 Archivo digital Producción Científica Artículos científicos Disponible Documentos electrónicos
2012-092.PDFAdobe Acrobat PDF
